| FALK, Martin, I FALKOVÁ, O KOPEČNÁ, A BAČÍKOVÁ, E PAGÁČOVÁ, D ŠIMEK, M GOLAN, Stanislav KOZUBEK, M PEKAROVÁ, Follett SE, B KLEJDUS, Elliott KW, K VARGA, O TEPLÁ and I KRATOCHVÍLOVÁ. Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants. Scientific reports. London: NATURE PUBLISHING GROUP, 2018, vol. 8, No 1, p. Article number 14694. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-018-32939-5. |
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@article{1594185,
author = {Falk, Martin and Falková, I and Kopečná, O and Bačíková, A and Pagáčová, E and Šimek, D and Golan, M and Kozubek, Stanislav and Pekarová, M and SE, Follett and Klejdus, B and KW, Elliott and Varga, K and Teplá, O and Kratochvílová, I},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-018-32939-5},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants},
url = {http://dx.doi.org/10.1038/s41598-018-32939-5},
volume = {8},
year = {2018}
}
TY - JOUR
ID - 1594185
AU - Falk, Martin - Falková, I - Kopečná, O - Bačíková, A - Pagáčová, E - Šimek, D - Golan, M - Kozubek, Stanislav - Pekarová, M - SE, Follett - Klejdus, B - KW, Elliott - Varga, K - Teplá, O - Kratochvílová, I
PY - 2018
TI - Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants
JF - Scientific reports
VL - 8
IS - 1
SP - Article number 14694
EP - Article number 14694
PB - NATURE PUBLISHING GROUP
SN - 20452322
UR - http://dx.doi.org/10.1038/s41598-018-32939-5
L2 - http://dx.doi.org/10.1038/s41598-018-32939-5
N2 - In this work, we shed new light on the highly debated issue of chromatin fragmentation in cryopreserved cells. Moreover, for the first time, we describe replicating cell-specific DNA damage and higher-order chromatin alterations after freezing and thawing. We identified DNA structural changes associated with the freeze-thaw process and correlated them with the viability of frozen and thawed cells. We simultaneously evaluated DNA defects and the higher-order chromatin structure of frozen and thawed cells with and without cryoprotectant treatment. We found that in replicating (S phase) cells, DNA was preferentially damaged by replication fork collapse, potentially leading to DNA double strand breaks (DSBs), which represent an important source of both genome instability and defects in epigenome maintenance. This induction of DNA defects by the freeze-thaw process was not prevented by any cryoprotectant studied. Both in replicating and non-replicating cells, freezing and thawing altered the chromatin structure in a cryoprotectant-dependent manner. Interestingly, cells with condensed chromatin, which was strongly stimulated by dimethyl sulfoxide (DMSO) prior to freezing had the highest rate of survival after thawing. Our results will facilitate the design of compounds and procedures to decrease injury to cryopreserved cells.
ER -
FALK, Martin, I FALKOVÁ, O KOPEČNÁ, A BAČÍKOVÁ, E PAGÁČOVÁ, D ŠIMEK, M GOLAN, Stanislav KOZUBEK, M PEKAROVÁ, Follett SE, B KLEJDUS, Elliott KW, K VARGA, O TEPLÁ and I KRATOCHVÍLOVÁ. Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants. \textit{Scientific reports}. London: NATURE PUBLISHING GROUP, 2018, vol.~8, No~1, p.~Article number 14694. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-018-32939-5.
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