Theoretical and experimental study of the antifreeze protein
AFP752, trehalose and dimethylsulfoxide cryoprotection
mechanism: correlation with cryopreserved cell viability

J 2017

Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ et. al.

Základní údaje

Originální název

Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

Autoři

KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ, J ŠEBERA, J MIČOVÁ, Martin FALK, I FALKOVÁ, D ŘEHA, K W ELLIOTT, K VARGA, S E FOLLETT a D ŠIMEK

Vydání

RSC Advances, Cambridge, Royal Society of Chemistry, 2017, 2046-2069

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.936

DOI

http://dx.doi.org/10.1039/c6ra25095e

UT WoS

000393741900048

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 12. 2019 13:42, doc. RNDr. Martin Falk, Ph.D.

Anotace

V originále

In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.

Návaznosti

GA16-12454S, projekt VaV

Název: Charakterizace a modifikace komplexní odpovědi buněk nádorů hlavy a krku na různá záření - krok kupředu ke kombinované personalizované (radio)terapii

Investor: Grantová agentura ČR, Charakterizace a modifikace komplexní odpovědi buněk nádorů hlavy a krku na různá záření - krok kupředu ke kombinované personalizované (radio)terapii

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

NV16-29835A, projekt VaV

Název: Molekulárně-genetické markery predikce účinnosti radioterapie u nádorů hlavy a krku

Citovat

KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ, J ŠEBERA, J MIČOVÁ, Martin FALK, I FALKOVÁ, D ŘEHA, K W ELLIOTT, K VARGA, S E FOLLETT a D ŠIMEK. Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability. RSC Advances. Cambridge: Royal Society of Chemistry, 2017, roč. 7, č. 1, s. 352-360. ISSN 2046-2069. Dostupné z: https://dx.doi.org/10.1039/c6ra25095e.

@article{1594210,
   author = {Kratochvílová, I and Golan, M and Pomeisl, K and Richter, J and Sedláková, S and Šebera, J and Mičová, J and Falk, Martin and Falková, I and Řeha, D and Elliott, K W and Varga, K and Follett, S E and Šimek, D},
   article_location = {Cambridge},
   article_number = {1},
   doi = {http://dx.doi.org/10.1039/c6ra25095e},
   language = {eng},
   issn = {2046-2069},
   journal = {RSC Advances},
   title = {Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability},
   url = {http://dx.doi.org/10.1039/c6ra25095e},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1594210
AU  - Kratochvílová, I - Golan, M - Pomeisl, K - Richter, J - Sedláková, S - Šebera, J - Mičová, J - Falk, Martin - Falková, I - Řeha, D - Elliott, K W - Varga, K - Follett, S E - Šimek, D
PY  - 2017
TI  - Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability
JF  - RSC Advances
VL  - 7
IS  - 1
SP  - 352-360
EP  - 352-360
PB  - Royal Society of Chemistry
SN  - 20462069
UR  - http://dx.doi.org/10.1039/c6ra25095e
L2  - http://dx.doi.org/10.1039/c6ra25095e
N2  - In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
ER  -

KRATOCHVÍLOVÁ, I, M GOLAN, K POMEISL, J RICHTER, S SEDLÁKOVÁ, J ŠEBERA, J MIČOVÁ, Martin FALK, I FALKOVÁ, D ŘEHA, K W ELLIOTT, K VARGA, S E FOLLETT a D ŠIMEK. Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability. \textit{RSC Advances}. Cambridge: Royal Society of Chemistry, 2017, roč.~7, č.~1, s.~352-360. ISSN~2046-2069. Dostupné z: https://dx.doi.org/10.1039/c6ra25095e.

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