Occurrence of Thermophilic Microorganisms in Different Full
Scale Biogas Plants

J 2020

Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

KUSHKEVYCH, Ivan, Jiří CEJNAR, Monika VÍTĚZOVÁ, Tomáš VÍTĚZ, Dani DORDEVIĆ et. al.

Basic information

Original name

Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

Authors

KUSHKEVYCH, Ivan (804 Ukraine, guarantor, belonging to the institution), Jiří CEJNAR (203 Czech Republic, belonging to the institution), Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution), Tomáš VÍTĚZ (203 Czech Republic), Dani DORDEVIĆ (203 Czech Republic) and Yannick BOMBLE (840 United States of America)

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14310/20:00115159

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms21010283

UT WoS

000515378000283

Keywords in English

renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters

Abstract

V originále

Background: In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modřice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Čejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants.

Links

MUNI/A/0902/2018, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 3

Investor: Masaryk University, Category A

MUNI/A/0947/2019, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 4 (Acronym: SV2020)

Investor: Masaryk University, Category A

Citovat

KUSHKEVYCH, Ivan, Jiří CEJNAR, Monika VÍTĚZOVÁ, Tomáš VÍTĚZ, Dani DORDEVIĆ and Yannick BOMBLE. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. International Journal of Molecular Sciences. Basel: MDPI, 2020, vol. 21, No 1, p. 1-12. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21010283.

@article{1596757,
   author = {Kushkevych, Ivan and Cejnar, Jiří and Vítězová, Monika and Vítěz, Tomáš and Dordević, Dani and Bomble, Yannick},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms21010283},
   keywords = {renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants},
   url = {https://www.mdpi.com/1422-0067/21/1/283},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1596757
AU  - Kushkevych, Ivan - Cejnar, Jiří - Vítězová, Monika - Vítěz, Tomáš - Dordević, Dani - Bomble, Yannick
PY  - 2020
TI  - Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 14220067
KW  - renewable energy
KW  - biogas
KW  - Illumina sequencing
KW  - thermophilic microorganisms
KW  - anaerobic digesters
UR  - https://www.mdpi.com/1422-0067/21/1/283
L2  - https://www.mdpi.com/1422-0067/21/1/283
N2  - Background: In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modřice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Čejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants.
ER  -

KUSHKEVYCH, Ivan, Jiří CEJNAR, Monika VÍTĚZOVÁ, Tomáš VÍTĚZ, Dani DORDEVI$\backslash$'C and Yannick BOMBLE. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~1, p.~1-12. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21010283.

Detailed Information on Publication Record