Novel synergistic culturing approach for efficient and robust
differentiation of human pluripotent stem cells into
endothelial progenitors.

a 2019

Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ, Irena KOUTNÁ et. al.

Základní údaje

Originální název

Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.

Autoři

FARKAŠ, Šimon (703 Slovensko, domácí), Pavel ŠIMARA (203 Česká republika, garant, domácí), Daniela ŘEHÁKOVÁ (203 Česká republika, domácí), Tereza SOURALOVÁ (203 Česká republika, domácí) a Irena KOUTNÁ (203 Česká republika, domácí)

Vydání

8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/19:00108561

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

pluripotentní kmenové buňky; endotheliální diferenciace

Klíčová slova anglicky

pluripotent stem cells; endothelial differentiation

Štítky

rivok

Změněno: 31. 5. 2023 12:56, Mgr. Tereza Souralová

Anotace

V originále

Cellular therapy is a powerful tool for treating various human diseases and reparation of the damaged vasculature is a specialized subtype of this therapy. Using human pluripotent stem cells (hPSCs) as a starting material, it should be possible to generate high amounts of endothelial progenitor cells (EPCs). Our aim was to develop highly efficient, robust and easily reproducible differentiation protocol that would allow us to produce these high amounts of EPCs. Presented protocol follows proper physiological pathway by differentiating hPSCs in three phases through primitive streak and kinase insert domain receptor (KDR) positive mesoderm into EPCs. The differentiation protocol only takes 5 days and results in high amounts of pure EPCs. The robustness is demonstrated by using both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Better efficiency and lesser line-to-line variability were observed for our differentiation protocol in comparison to other published protocols. Furthermore, derived EPCs expressed high proliferative potential EPC marker CD157 on their surface in addition to standard EPC markers CD31, CD144, CD34, KDR and C-X-C chemokine receptor type 4 (CXCR4).

Návaznosti

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ a Irena KOUTNÁ. Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors. In 8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic. 2019.

@proceedings{1599660,
   author = {Farkaš, Šimon and Šimara, Pavel and Řeháková, Daniela and Souralová, Tereza and Koutná, Irena},
   booktitle = {8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic},
   keywords = {pluripotent stem cells; endothelial differentiation},
   language = {eng},
   title = {Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.},
   year = {2019}
}

TY  - CONF
ID  - 1599660
AU  - Farkaš, Šimon - Šimara, Pavel - Řeháková, Daniela - Souralová, Tereza - Koutná, Irena
PY  - 2019
TI  - Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.
KW  - pluripotent stem cells
KW  - endothelial differentiation
N2  - Cellular therapy is a powerful tool for treating various human diseases and reparation of the damaged vasculature is a specialized subtype of this therapy. Using human pluripotent stem cells (hPSCs) as a starting material, it should be possible to generate high amounts of endothelial progenitor cells (EPCs). Our aim was to develop highly efficient, robust and easily reproducible differentiation protocol that would allow us to produce these high amounts of EPCs. Presented protocol follows proper physiological pathway by differentiating hPSCs in three phases through primitive streak and kinase insert domain receptor (KDR) positive mesoderm into EPCs. The differentiation protocol only takes 5 days and results in high amounts of pure EPCs. The robustness is demonstrated by using both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Better efficiency and lesser line-to-line variability were observed for our differentiation protocol in comparison to other published protocols. Furthermore, derived EPCs expressed high proliferative potential EPC marker CD157 on their surface in addition to standard EPC markers CD31, CD144, CD34, KDR and C-X-C chemokine receptor type 4 (CXCR4).
ER  -

FARKAŠ, Šimon, Pavel ŠIMARA, Daniela ŘEHÁKOVÁ, Tereza SOURALOVÁ a Irena KOUTNÁ. Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors. In \textit{8. ročník konference Stem Cells and Cell Therapy: From Research to Modern Clinical Applications, Lednice, Czech Republic}. 2019.

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