Different Modes of Action of Genetic and Chemical
Downregulation of Histone Deacetylases with Respect to Plant
Development and Histone Modifications

J 2019

Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ et. al.

Základní údaje

Originální název

Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications

Autoři

LOCHMANOVÁ, Gabriela (203 Česká republika, domácí), Ivana IHNATOVÁ (703 Slovensko, domácí), Hana KUCHAŘÍKOVÁ (203 Česká republika, domácí), Sylva BRABENCOVÁ (203 Česká republika, domácí), Dagmar ZACHOVÁ (203 Česká republika, domácí), Jiří FAJKUS (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí) a Miloslava FOJTOVÁ (203 Česká republika, domácí)

Vydání

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.556

Kód RIV

RIV/00216224:14740/19:00112144

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/ijms20205093

UT WoS

000498822800125

Klíčová slova anglicky

Arabidopsis thaliana; epigenetics; histone; mass spectrometry; post-translational modifications; sodiumbutyrate; trichostatin A

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:23, Ing. Martina Blahová

Anotace

V originále

A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL a Miloslava FOJTOVÁ. Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019, roč. 20, č. 20, s. "5093", 14 s. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms20205093.

@article{1600503,
   author = {Lochmanová, Gabriela and Ihnatová, Ivana and Kuchaříková, Hana and Brabencová, Sylva and Zachová, Dagmar and Fajkus, Jiří and Zdráhal, Zbyněk and Fojtová, Miloslava},
   article_number = {20},
   doi = {http://dx.doi.org/10.3390/ijms20205093},
   keywords = {Arabidopsis thaliana; epigenetics; histone; mass spectrometry; post-translational modifications; sodiumbutyrate; trichostatin A},
   language = {eng},
   issn = {1422-0067},
   journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
   title = {Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications},
   url = {https://www.mdpi.com/1422-0067/20/20/5093},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1600503
AU  - Lochmanová, Gabriela - Ihnatová, Ivana - Kuchaříková, Hana - Brabencová, Sylva - Zachová, Dagmar - Fajkus, Jiří - Zdráhal, Zbyněk - Fojtová, Miloslava
PY  - 2019
TI  - Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
JF  - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
VL  - 20
IS  - 20
SP  - "5093"
EP  - "5093"
SN  - 14220067
KW  - Arabidopsis thaliana
KW  - epigenetics
KW  - histone
KW  - mass spectrometry
KW  - post-translational modifications
KW  - sodiumbutyrate
KW  - trichostatin A
UR  - https://www.mdpi.com/1422-0067/20/20/5093
L2  - https://www.mdpi.com/1422-0067/20/20/5093
N2  - A high degree of developmental plasticity enables plants to adapt to continuous, often unfavorable and unpredictable changes in their environment. At the molecular level, adaptive advantages for plants are primarily provided by epigenetic machinery including DNA methylation, histone modifications, and the activity of noncoding RNA molecules. Using a mass spectrometry-based proteomic approach, we examined the levels of acetylated histone peptide forms in Arabidopsis plants with a loss of function of histone deacetylase 6 (HDA6), and in plants germinated in the presence of HDA inhibitors trichostatin A (TSA) and sodium butyrate (NaB). Our analyses revealed particular lysine sites at histone sequences targeted by the HDA6 enzyme, and by TSA- and NaB-sensitive HDAs. Compared with plants exposed to drugs, more dramatic changes in the overall profiles of histone post-translational modifications were identified in hda6 mutants. However, loss of HDA6 was not sufficient by itself to induce hyperacetylation to the maximum degree, implying complementary activities of other HDAs. In contrast to hda6 mutants that did not exhibit any obvious phenotypic defects, the phenotypes of seedlings exposed to HDA inhibitors were markedly affected, showing that the effect of these drugs on early plant development is not limited to the modulation of histone acetylation levels.
ER  -

LOCHMANOVÁ, Gabriela, Ivana IHNATOVÁ, Hana KUCHAŘÍKOVÁ, Sylva BRABENCOVÁ, Dagmar ZACHOVÁ, Jiří FAJKUS, Zbyněk ZDRÁHAL a Miloslava FOJTOVÁ. Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications. \textit{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}. 2019, roč.~20, č.~20, s.~''5093'', 14 s. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms20205093.

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