DNA Damage Changes Distribution Pattern and Levels of HP1
Protein Isoforms in the Nucleolus and Increases Phosphorylation
of HP1 beta-Ser88

J 2019

DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88

LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ, Zbyněk ZDRÁHAL, Stanislav KOZUBEK, Jiří ŠPONER et. al.

Základní údaje

Originální název

DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88

Autoři

LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), Stanislav KOZUBEK (203 Česká republika), Jiří ŠPONER (203 Česká republika), Miroslav KREPL (203 Česká republika), Pavlína POKORNÁ (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

CELLS, 2019, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.366

Kód RIV

RIV/00216224:14740/19:00107962

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/cells8091097

UT WoS

000489103800156

Klíčová slova anglicky

HP1; irradiation; nucleolus; phosphorylation; mass spectrometry; epigenetics; FLIM-FRET

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2024 14:11, Ing. Martina Blahová

Anotace

V originále

The family of heterochromatin protein 1 (HP1) isoforms is essential for chromatin packaging, regulation of gene expression, and repair of damaged DNA. Here we document that gamma-radiation reduced the number of HP1 alpha-positive foci, but not HP1 beta and HP1 gamma foci, located in the vicinity of the fibrillarin-positive region of the nucleolus. The additional analysis confirmed that gamma-radiation has the ability to significantly decrease the level of HP1 alpha in rDNA promoter and rDNA encoding 28S rRNA. By mass spectrometry, we showed that treatment by gamma-rays enhanced the HP1 beta serine 88 phosphorylation (S88ph), but other analyzed modifications of HP1 beta, including S161ph/Y163ph, S171ph, and S174ph, were not changed in cells exposed to gamma-rays or treated by the HDAC inhibitor (HDACi). Interestingly, a combination of HDACi and gamma-radiation increased the level of HP1 alpha and HP1 gamma. The level of HP1 beta remained identical before and after the HDACi/gamma-rays treatment, but HDACi strengthened HP1 beta interaction with the KRAB-associated protein 1 (KAP1) protein. Conversely, HP1 gamma did not interact with KAP1, although approximately 40% of HP1 gamma foci co-localized with accumulated KAP1. Especially HP1 gamma foci at the periphery of nucleoli were mostly absent of KAP1. Together, DNA damage changed the morphology, levels, and interaction properties of HP1 isoforms. Also, gamma-irradiation-induced hyperphosphorylation of the HP1 beta protein; thus, HP1 beta-S88ph could be considered as an important marker of DNA damage.

Návaznosti

GA18-07384S, projekt VaV

Název: Od konformace po biologické funkce proteinu HP1

Investor: Grantová agentura ČR, Od konformace po biologické funkce proteinu HP1

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ, Zbyněk ZDRÁHAL, Stanislav KOZUBEK, Jiří ŠPONER, Miroslav KREPL, Pavlína POKORNÁ a Eva BÁRTOVÁ. DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88. CELLS. 2019, roč. 8, č. 9, s. "1097", 22 s. ISSN 2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells8091097.

@article{1600517,
   author = {Legartová, Sofia and Lochmanová, Gabriela and Zdráhal, Zbyněk and Kozubek, Stanislav and Šponer, Jiří and Krepl, Miroslav and Pokorná, Pavlína and Bártová, Eva},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/cells8091097},
   keywords = {HP1; irradiation; nucleolus; phosphorylation; mass spectrometry; epigenetics; FLIM-FRET},
   language = {eng},
   issn = {2073-4409},
   journal = {CELLS},
   title = {DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88},
   url = {https://www.mdpi.com/2073-4409/8/9/1097},
   volume = {8},
   year = {2019}
}

TY  - JOUR
ID  - 1600517
AU  - Legartová, Sofia - Lochmanová, Gabriela - Zdráhal, Zbyněk - Kozubek, Stanislav - Šponer, Jiří - Krepl, Miroslav - Pokorná, Pavlína - Bártová, Eva
PY  - 2019
TI  - DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88
JF  - CELLS
VL  - 8
IS  - 9
SP  - "1097"
EP  - "1097"
SN  - 20734409
KW  - HP1
KW  - irradiation
KW  - nucleolus
KW  - phosphorylation
KW  - mass spectrometry
KW  - epigenetics
KW  - FLIM-FRET
UR  - https://www.mdpi.com/2073-4409/8/9/1097
L2  - https://www.mdpi.com/2073-4409/8/9/1097
N2  - The family of heterochromatin protein 1 (HP1) isoforms is essential for chromatin packaging, regulation of gene expression, and repair of damaged DNA. Here we document that gamma-radiation reduced the number of HP1 alpha-positive foci, but not HP1 beta and HP1 gamma foci, located in the vicinity of the fibrillarin-positive region of the nucleolus. The additional analysis confirmed that gamma-radiation has the ability to significantly decrease the level of HP1 alpha in rDNA promoter and rDNA encoding 28S rRNA. By mass spectrometry, we showed that treatment by gamma-rays enhanced the HP1 beta serine 88 phosphorylation (S88ph), but other analyzed modifications of HP1 beta, including S161ph/Y163ph, S171ph, and S174ph, were not changed in cells exposed to gamma-rays or treated by the HDAC inhibitor (HDACi). Interestingly, a combination of HDACi and gamma-radiation increased the level of HP1 alpha and HP1 gamma. The level of HP1 beta remained identical before and after the HDACi/gamma-rays treatment, but HDACi strengthened HP1 beta interaction with the KRAB-associated protein 1 (KAP1) protein. Conversely, HP1 gamma did not interact with KAP1, although approximately 40% of HP1 gamma foci co-localized with accumulated KAP1. Especially HP1 gamma foci at the periphery of nucleoli were mostly absent of KAP1. Together, DNA damage changed the morphology, levels, and interaction properties of HP1 isoforms. Also, gamma-irradiation-induced hyperphosphorylation of the HP1 beta protein; thus, HP1 beta-S88ph could be considered as an important marker of DNA damage.
ER  -

LEGARTOVÁ, Sofia, Gabriela LOCHMANOVÁ, Zbyněk ZDRÁHAL, Stanislav KOZUBEK, Jiří ŠPONER, Miroslav KREPL, Pavlína POKORNÁ a Eva BÁRTOVÁ. DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88. \textit{CELLS}. 2019, roč.~8, č.~9, s.~''1097'', 22 s. ISSN~2073-4409. Dostupné z: https://dx.doi.org/10.3390/cells8091097.

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