A public database for the new MLST scheme for Treponema
pallidum subsp. pallidum: surveillance and epidemiology of the
causative agent of syphilis

J 2019

A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS a Mathieu PICARDEAU

Základní údaje

Originální název

A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis

Autoři

GRILLOVÁ, Linda (203 Česká republika, garant), Keith JOLLEY (826 Velká Británie a Severní Irsko), David ŠMAJS (203 Česká republika, domácí) a Mathieu PICARDEAU (250 Francie)

Vydání

PeerJ, London, PEERJ INC, 2019, 2167-8359

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.379

Kód RIV

RIV/00216224:14110/19:00108563

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.7717/peerj.6182

UT WoS

000455261800007

Klíčová slova anglicky

Treponema pallidum subsp. pallidum; Molecular typing; PubMLST

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2020 14:05, Mgr. Tereza Miškechová

Anotace

V originále

Treponema pallidum subsp. pallidum is the causative agent of syphilis, a sexually transmitted disease with worldwide prevalence. Several different molecular typing schemes are currently available for this pathogen. To enable population biology studies of the syphilis agent and for epidemiological surveillance at the global scale, a harmonized typing tool needs to be introduced. Recently, we published a new multi-locus sequence typing (MLST) with the potential to significantly enhance the epidemiological data in several aspects (e.g., distinguishing genetically different clades of syphilis, subtyping inside these clades, and finally, distinguishing different subspecies of non-cultivable pathogenic treponemes). In this short report, we introduce the PubMLST database for treponemal DNA data storage and for assignments of allelic profiles and sequencing types. Moreover, we have summarized epidemiological data of all treponemal strains (n = 358) with available DNA sequences in typing loci and found several association between genetic groups and characteristics of patients. This study proposes the establishment of a single MLST of T. p. pallidum and encourages researchers and public health communities to use this PubMLST database as a universal tool for molecular typing studies of the syphilis pathogen.

Návaznosti

MUNI/A/1087/2018, interní kód MU

Název: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masarykova univerzita, Molekulární a buněčná biologie pro biomedicínské vědy, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV17-31333A, projekt VaV

Název: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS a Mathieu PICARDEAU. A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis. PeerJ. London: PEERJ INC, 2019, roč. 6, JAN 9 2019, s. 1-13. ISSN 2167-8359. Dostupné z: https://dx.doi.org/10.7717/peerj.6182.

@article{1603885,
   author = {Grillová, Linda and Jolley, Keith and Šmajs, David and Picardeau, Mathieu},
   article_location = {London},
   article_number = {JAN 9 2019},
   doi = {http://dx.doi.org/10.7717/peerj.6182},
   keywords = {Treponema pallidum subsp. pallidum; Molecular typing; PubMLST},
   language = {eng},
   issn = {2167-8359},
   journal = {PeerJ},
   title = {A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis},
   url = {http://dx.doi.org/10.7717/peerj.6182},
   volume = {6},
   year = {2019}
}

TY  - JOUR
ID  - 1603885
AU  - Grillová, Linda - Jolley, Keith - Šmajs, David - Picardeau, Mathieu
PY  - 2019
TI  - A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis
JF  - PeerJ
VL  - 6
IS  - JAN 9 2019
SP  - 1-13
EP  - 1-13
PB  - PEERJ INC
SN  - 21678359
KW  - Treponema pallidum subsp. pallidum
KW  - Molecular typing
KW  - PubMLST
UR  - http://dx.doi.org/10.7717/peerj.6182
L2  - http://dx.doi.org/10.7717/peerj.6182
N2  - Treponema pallidum subsp. pallidum is the causative agent of syphilis, a sexually transmitted disease with worldwide prevalence. Several different molecular typing schemes are currently available for this pathogen. To enable population biology studies of the syphilis agent and for epidemiological surveillance at the global scale, a harmonized typing tool needs to be introduced. Recently, we published a new multi-locus sequence typing (MLST) with the potential to significantly enhance the epidemiological data in several aspects (e.g., distinguishing genetically different clades of syphilis, subtyping inside these clades, and finally, distinguishing different subspecies of non-cultivable pathogenic treponemes). In this short report, we introduce the PubMLST database for treponemal DNA data storage and for assignments of allelic profiles and sequencing types. Moreover, we have summarized epidemiological data of all treponemal strains (n = 358) with available DNA sequences in typing loci and found several association between genetic groups and characteristics of patients. This study proposes the establishment of a single MLST of T. p. pallidum and encourages researchers and public health communities to use this PubMLST database as a universal tool for molecular typing studies of the syphilis pathogen.
ER  -

GRILLOVÁ, Linda, Keith JOLLEY, David ŠMAJS a Mathieu PICARDEAU. A public database for the new MLST scheme for Treponema pallidum subsp. pallidum: surveillance and epidemiology of the causative agent of syphilis. \textit{PeerJ}. London: PEERJ INC, 2019, roč.~6, JAN 9 2019, s.~1-13. ISSN~2167-8359. Dostupné z: https://dx.doi.org/10.7717/peerj.6182.

Podrobný výpis o publikaci