Obesity-induced nucleosome release predicts poor
cardio-metabolic health

J 2019

Obesity-induced nucleosome release predicts poor cardio-metabolic health

LO RE, Oriana, Andrea MAUGERI, Jana HRUŠKOVÁ, Juraj JAKUBÍK, Jan KUČERA et. al.

Základní údaje

Originální název

Obesity-induced nucleosome release predicts poor cardio-metabolic health

Autoři

LO RE, Oriana (380 Itálie, domácí), Andrea MAUGERI (380 Itálie), Jana HRUŠKOVÁ (203 Česká republika), Juraj JAKUBÍK (703 Slovensko), Jan KUČERA (203 Česká republika, domácí), Julie DOBROVOLNÁ (203 Česká republika, domácí), Jude A. OBEN (826 Velká Británie a Severní Irsko), Lukáš KUBALA (203 Česká republika, domácí), Adéla DVOŘÁKOVÁ (203 Česká republika, domácí), Milan ČÍŽ (203 Česká republika, domácí) a Manlio VINCIGUERRA (380 Itálie, garant)

Vydání

CLINICAL EPIGENETICS, LONDON, BMC, 2019, 1868-7075

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30101 Human genetics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.028

Kód RIV

RIV/00216224:14110/19:00112410

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1186/s13148-019-0797-8

UT WoS

000505032600001

Klíčová slova anglicky

Liquid biopsy; Nucleosome; Epigenetics; Metabolic health; Cardiovascular disease

Štítky

14110513, 14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 2. 2023 11:31, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Objective While circulating nucleosome levels are high in obese mouse models, it is unknown where these nucleosomes originate from and whether they are a marker of cardio-metabolic health in humans. Here, we aimed to determine whether an association exists between circulating nucleosomes and the risk of developing obesity, metabolic syndrome (MetS) and/or a dysfunctional cardiovascular performance. Methods We randomly selected 120 participants of the Kardiovize Brno 2030 study across three BMI strata: BMI 18-25, 25-30, and > 30. We assessed the association between circulating nucleosome levels and the risk of obesity, MetS, and poor cardiovascular health. We then cultured human neutrophils, adipocytes, and hepatoma cells to study nucleosome origins in a fat-rich environment. Results Circulating nucleosome levels positively correlated with BMI (R = 0.602, p < 0.05), fatty liver index (R = 0.622, p < 0.05), left ventricular mass (R = 0.457, p < 0.05), and associated with MetS (p < 0.001) and poor cardiovascular health (p < 0.001). Incubating neutrophils with 1-10 mu M free fatty acids triggered nucleosome production without concomitant cell death. Nucleosomes were not produced during pre-adipocyte differentiation or upon incubation of hepatic cells with palmitic acid. Conclusions Neutrophils are a bona fide source of circulating nucleosomes in an obesogenic environment and in overweight/obese patients. High nucleosome levels are associated with MetS and cardiovascular performance, and might represent novel candidate biomarkers for cardio-metabolic health.

Návaznosti

MUNI/A/1553/2018, interní kód MU

Název: Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí (Akronym: genetika; stres; biomateriály; tkáňové kultury)

Investor: Masarykova univerzita, Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

LO RE, Oriana, Andrea MAUGERI, Jana HRUŠKOVÁ, Juraj JAKUBÍK, Jan KUČERA, Julie DOBROVOLNÁ, Jude A. OBEN, Lukáš KUBALA, Adéla DVOŘÁKOVÁ, Milan ČÍŽ a Manlio VINCIGUERRA. Obesity-induced nucleosome release predicts poor cardio-metabolic health. CLINICAL EPIGENETICS. LONDON: BMC, 2019, roč. 12, č. 1, s. 1-14. ISSN 1868-7075. Dostupné z: https://dx.doi.org/10.1186/s13148-019-0797-8.

@article{1604540,
   author = {Lo Re, Oriana and Maugeri, Andrea and Hrušková, Jana and Jakubík, Juraj and Kučera, Jan and Dobrovolná, Julie and Oben, Jude A. and Kubala, Lukáš and Dvořáková, Adéla and Číž, Milan and Vinciguerra, Manlio},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s13148-019-0797-8},
   keywords = {Liquid biopsy; Nucleosome; Epigenetics; Metabolic health; Cardiovascular disease},
   language = {eng},
   issn = {1868-7075},
   journal = {CLINICAL EPIGENETICS},
   title = {Obesity-induced nucleosome release predicts poor cardio-metabolic health},
   url = {http://dx.doi.org/10.1186/s13148-019-0797-8},
   volume = {12},
   year = {2019}
}

TY  - JOUR
ID  - 1604540
AU  - Lo Re, Oriana - Maugeri, Andrea - Hrušková, Jana - Jakubík, Juraj - Kučera, Jan - Dobrovolná, Julie - Oben, Jude A. - Kubala, Lukáš - Dvořáková, Adéla - Číž, Milan - Vinciguerra, Manlio
PY  - 2019
TI  - Obesity-induced nucleosome release predicts poor cardio-metabolic health
JF  - CLINICAL EPIGENETICS
VL  - 12
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - BMC
SN  - 18687075
KW  - Liquid biopsy
KW  - Nucleosome
KW  - Epigenetics
KW  - Metabolic health
KW  - Cardiovascular disease
UR  - http://dx.doi.org/10.1186/s13148-019-0797-8
L2  - http://dx.doi.org/10.1186/s13148-019-0797-8
N2  - Objective While circulating nucleosome levels are high in obese mouse models, it is unknown where these nucleosomes originate from and whether they are a marker of cardio-metabolic health in humans. Here, we aimed to determine whether an association exists between circulating nucleosomes and the risk of developing obesity, metabolic syndrome (MetS) and/or a dysfunctional cardiovascular performance. Methods We randomly selected 120 participants of the Kardiovize Brno 2030 study across three BMI strata: BMI 18-25, 25-30, and > 30. We assessed the association between circulating nucleosome levels and the risk of obesity, MetS, and poor cardiovascular health. We then cultured human neutrophils, adipocytes, and hepatoma cells to study nucleosome origins in a fat-rich environment. Results Circulating nucleosome levels positively correlated with BMI (R = 0.602, p < 0.05), fatty liver index (R = 0.622, p < 0.05), left ventricular mass (R = 0.457, p < 0.05), and associated with MetS (p < 0.001) and poor cardiovascular health (p < 0.001). Incubating neutrophils with 1-10 mu M free fatty acids triggered nucleosome production without concomitant cell death. Nucleosomes were not produced during pre-adipocyte differentiation or upon incubation of hepatic cells with palmitic acid. Conclusions Neutrophils are a bona fide source of circulating nucleosomes in an obesogenic environment and in overweight/obese patients. High nucleosome levels are associated with MetS and cardiovascular performance, and might represent novel candidate biomarkers for cardio-metabolic health.
ER  -

LO RE, Oriana, Andrea MAUGERI, Jana HRUŠKOVÁ, Juraj JAKUBÍK, Jan KUČERA, Julie DOBROVOLNÁ, Jude A. OBEN, Lukáš KUBALA, Adéla DVOŘÁKOVÁ, Milan ČÍŽ a Manlio VINCIGUERRA. Obesity-induced nucleosome release predicts poor cardio-metabolic health. \textit{CLINICAL EPIGENETICS}. LONDON: BMC, 2019, roč.~12, č.~1, s.~1-14. ISSN~1868-7075. Dostupné z: https://dx.doi.org/10.1186/s13148-019-0797-8.

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