SKUBALOVA, Zuzana, Hana MICHALKOVA, Petr MICHALEK, Vladislav STRMISKA, Roman GURAN, Miguel Angel Merlos RODRIGO, Klara CASTKOVA, David HYNEK, Vladimír PEKAŘÍK, Ondrej ZITKA, Vojtech ADAM a Zbynek HEGER. Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloids and Surfaces B: Biointerfaces. Amsterdam: Elsevier, 2019, roč. 182, OCT 1 2019, s. 1-10. ISSN 0927-7765. Dostupné z: https://dx.doi.org/10.1016/j.colsurfb.2019.110391. |
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@article{1604897, author = {Skubalova, Zuzana and Michalkova, Hana and Michalek, Petr and Strmiska, Vladislav and Guran, Roman and Rodrigo, Miguel Angel Merlos and Castkova, Klara and Hynek, David and Pekařík, Vladimír and Zitka, Ondrej and Adam, Vojtech and Heger, Zbynek}, article_location = {Amsterdam}, article_number = {OCT 1 2019}, doi = {http://dx.doi.org/10.1016/j.colsurfb.2019.110391}, keywords = {Cytotoxicity; Nanotoxicology; Oxidative stress; Proteomics; Titanium}, language = {eng}, issn = {0927-7765}, journal = {Colloids and Surfaces B: Biointerfaces}, title = {Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells}, url = {http://dx.doi.org/10.1016/j.colsurfb.2019.110391}, volume = {182}, year = {2019} }
TY - JOUR ID - 1604897 AU - Skubalova, Zuzana - Michalkova, Hana - Michalek, Petr - Strmiska, Vladislav - Guran, Roman - Rodrigo, Miguel Angel Merlos - Castkova, Klara - Hynek, David - Pekařík, Vladimír - Zitka, Ondrej - Adam, Vojtech - Heger, Zbynek PY - 2019 TI - Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells JF - Colloids and Surfaces B: Biointerfaces VL - 182 IS - OCT 1 2019 SP - 1-10 EP - 1-10 PB - Elsevier SN - 09277765 KW - Cytotoxicity KW - Nanotoxicology KW - Oxidative stress KW - Proteomics KW - Titanium UR - http://dx.doi.org/10.1016/j.colsurfb.2019.110391 L2 - http://dx.doi.org/10.1016/j.colsurfb.2019.110391 N2 - Nanoparticular form of titanium dioxide (TiO2 NPs) belongs to important industrial material. Despite being widely used, serious contradictions regarding biosafety of TiO2 NPs remain. We anticipate that such discrepancies could be due to a lack of understanding of a linkage between TiO2 NPs phase composition and cytotoxicity. Therefore, we synthesized two types of biphasic TiO2 NPs differing in an anatase-brookite phase composition. The study presents an array of in vitro data suggesting that TiO2 NPs with a prevailing anatase phase composition possess higher cytotoxicity compared to TiO2 NPs with an equal anatase-brookite crystallinity. This phenomenon was evidenced by significantly higher inhibition of metabolic activity and growth of epithelial and neuroblast-like cells. Moreover, anatase-prevailing TiO2 NPs tend to produce higher amount of reactive oxygen species resulting in DNA fragmentation. Further insights into the molecular aspects of cytotoxicity of anatase-prevailing TiO2 NPs were obtained by comparative proteomics delineating that TiO2 NPs deregulate expression of a variety of proteins and associated pathways. This inevitably results in a decreased cellular ability to detoxify reactive oxygen species and respond to various stress conditions. The study provides novel data that add another piece to the jigsaw of the relation between structural features of NPs and biosafety. ER -
SKUBALOVA, Zuzana, Hana MICHALKOVA, Petr MICHALEK, Vladislav STRMISKA, Roman GURAN, Miguel Angel Merlos RODRIGO, Klara CASTKOVA, David HYNEK, Vladimír PEKAŘÍK, Ondrej ZITKA, Vojtech ADAM a Zbynek HEGER. Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. \textit{Colloids and Surfaces B: Biointerfaces}. Amsterdam: Elsevier, 2019, roč.~182, OCT 1 2019, s.~1-10. ISSN~0927-7765. Dostupné z: https://dx.doi.org/10.1016/j.colsurfb.2019.110391.
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