Generation and characterization of DSPP-Cerulean/DMP1-Cherry
reporter mice

J 2019

Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice

VIJAYKUMAR, Anushree, Sean GHASSEM-ZADEH, Ivana VIDOVIC-ZDRILIC, Karren KOMITAS, Igor ADAMEYKO et. al.

Základní údaje

Originální název

Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice

Autoři

VIJAYKUMAR, Anushree (840 Spojené státy), Sean GHASSEM-ZADEH (840 Spojené státy), Ivana VIDOVIC-ZDRILIC (840 Spojené státy), Karren KOMITAS (840 Spojené státy), Igor ADAMEYKO (752 Švédsko), Jan KŘIVÁNEK (203 Česká republika, domácí), Yu FU (840 Spojené státy), Peter MAYE (840 Spojené státy) a Mina MINA (840 Spojené státy, garant)

Vydání

GENESIS, MALDEN, WILEY-BLACKWELL, 2019, 1526-954X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.760

Kód RIV

RIV/00216224:14110/19:00112451

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/dvg.23324

UT WoS

000489695700005

Klíčová slova anglicky

bone; dentin matrix protein 1; dentin sialophosphoprotein; fluorescent protein reporters; Odontoblasts

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 1. 2020 10:57, Mgr. Tereza Miškechová

Anotace

V originále

To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP-Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblasts.

Citovat

VIJAYKUMAR, Anushree, Sean GHASSEM-ZADEH, Ivana VIDOVIC-ZDRILIC, Karren KOMITAS, Igor ADAMEYKO, Jan KŘIVÁNEK, Yu FU, Peter MAYE a Mina MINA. Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice. GENESIS. MALDEN: WILEY-BLACKWELL, 2019, roč. 57, č. 10, s. 1-11. ISSN 1526-954X. Dostupné z: https://dx.doi.org/10.1002/dvg.23324.

@article{1604919,
   author = {Vijaykumar, Anushree and GhassemandZadeh, Sean and VidovicandZdrilic, Ivana and Komitas, Karren and Adameyko, Igor and Křivánek, Jan and Fu, Yu and Maye, Peter and Mina, Mina},
   article_location = {MALDEN},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/dvg.23324},
   keywords = {bone; dentin matrix protein 1; dentin sialophosphoprotein; fluorescent protein reporters; Odontoblasts},
   language = {eng},
   issn = {1526-954X},
   journal = {GENESIS},
   title = {Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice},
   url = {http://dx.doi.org/10.1002/dvg.23324},
   volume = {57},
   year = {2019}
}

TY  - JOUR
ID  - 1604919
AU  - Vijaykumar, Anushree - Ghassem-Zadeh, Sean - Vidovic-Zdrilic, Ivana - Komitas, Karren - Adameyko, Igor - Křivánek, Jan - Fu, Yu - Maye, Peter - Mina, Mina
PY  - 2019
TI  - Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice
JF  - GENESIS
VL  - 57
IS  - 10
SP  - 1-11
EP  - 1-11
PB  - WILEY-BLACKWELL
SN  - 1526954X
KW  - bone
KW  - dentin matrix protein 1
KW  - dentin sialophosphoprotein
KW  - fluorescent protein reporters
KW  - Odontoblasts
UR  - http://dx.doi.org/10.1002/dvg.23324
L2  - http://dx.doi.org/10.1002/dvg.23324
N2  - To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP-Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblasts.
ER  -

VIJAYKUMAR, Anushree, Sean GHASSEM-ZADEH, Ivana VIDOVIC-ZDRILIC, Karren KOMITAS, Igor ADAMEYKO, Jan KŘIVÁNEK, Yu FU, Peter MAYE a Mina MINA. Generation and characterization of DSPP-Cerulean/DMP1-Cherry reporter mice. \textit{GENESIS}. MALDEN: WILEY-BLACKWELL, 2019, roč.~57, č.~10, s.~1-11. ISSN~1526-954X. Dostupné z: https://dx.doi.org/10.1002/dvg.23324.

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