Accuracy of PCR and serological testing for the diagnosis of
primary syphilis: Both tests are necessary

J 2019

Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary

NODA, Angel A., Islay RODRIGUEZ, Linda GRILLOVÁ, Philipp P. BOSSHARD, Reto LIENHARD et. al.

Základní údaje

Originální název

Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary

Autoři

NODA, Angel A. (192 Kuba, garant), Islay RODRIGUEZ (192 Kuba), Linda GRILLOVÁ (203 Česká republika, domácí), Philipp P. BOSSHARD (756 Švýcarsko) a Reto LIENHARD (756 Švýcarsko)

Vydání

INTERNATIONAL JOURNAL OF STD & AIDS, LONDON, SAGE PUBLICATIONS LTD, 2019, 0956-4624

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30303 Infectious Diseases

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.406

Kód RIV

RIV/00216224:14110/19:00112504

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1177/0956462419859764

UT WoS

000490799700001

Klíčová slova anglicky

Syphilis diagnosis; PCR; serology

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 1. 2020 10:40, Mgr. Tereza Miškechová

Anotace

V originále

Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid and accurate diagnosis is crucial to ensure patients and their contacts receive timely treatment to eradicate infection and prevent further transmission. In this prospective observational study, we evaluated the performance of polymerase chain reaction (PCR) and serological testing for the diagnosis of primary syphilis by evaluating anogenital swabs and sera from 178 Cuban patients presenting with ulcers. Three different PCR assays were evaluated targeting polA, tpp47 and 16S rDNA loci. Sera were evaluated with venereal disease research laboratory (VDRL) and T. pallidum hemagglutination (TPHA) assays. Assuming both methods were confirmatory, our data showed that PCR and serology did not correlate well (agreement = 52.3%, kappa 0.0512, 95% CI -0.0928-0.1951, p = 0.496). The sensitivities, specificities, positive and negative predictive values of the PCR assays were 76.1%, 100%, 100% and 57.9%, respectively, while the values for serology were 62.5%, 100%, 100% and 45.2%, respectively. The combination of PCR and serology can offer valuable information for the diagnosis of syphilis in patients presenting with anogenital ulceration avoiding further clinical complications and disease transmission.

Citovat

NODA, Angel A., Islay RODRIGUEZ, Linda GRILLOVÁ, Philipp P. BOSSHARD a Reto LIENHARD. Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary. INTERNATIONAL JOURNAL OF STD & AIDS. LONDON: SAGE PUBLICATIONS LTD, 2019, roč. 30, č. 11, s. 1087-1094. ISSN 0956-4624. Dostupné z: https://dx.doi.org/10.1177/0956462419859764.

@article{1605463,
   author = {Noda, Angel A. and Rodriguez, Islay and Grillová, Linda and Bosshard, Philipp P. and Lienhard, Reto},
   article_location = {LONDON},
   article_number = {11},
   doi = {http://dx.doi.org/10.1177/0956462419859764},
   keywords = {Syphilis diagnosis; PCR; serology},
   language = {eng},
   issn = {0956-4624},
   journal = {INTERNATIONAL JOURNAL OF STD & AIDS},
   title = {Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary},
   url = {http://dx.doi.org/10.1177/0956462419859764},
   volume = {30},
   year = {2019}
}

TY  - JOUR
ID  - 1605463
AU  - Noda, Angel A. - Rodriguez, Islay - Grillová, Linda - Bosshard, Philipp P. - Lienhard, Reto
PY  - 2019
TI  - Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary
JF  - INTERNATIONAL JOURNAL OF STD & AIDS
VL  - 30
IS  - 11
SP  - 1087-1094
EP  - 1087-1094
PB  - SAGE PUBLICATIONS LTD
SN  - 09564624
KW  - Syphilis diagnosis
KW  - PCR
KW  - serology
UR  - http://dx.doi.org/10.1177/0956462419859764
L2  - http://dx.doi.org/10.1177/0956462419859764
N2  - Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid and accurate diagnosis is crucial to ensure patients and their contacts receive timely treatment to eradicate infection and prevent further transmission. In this prospective observational study, we evaluated the performance of polymerase chain reaction (PCR) and serological testing for the diagnosis of primary syphilis by evaluating anogenital swabs and sera from 178 Cuban patients presenting with ulcers. Three different PCR assays were evaluated targeting polA, tpp47 and 16S rDNA loci. Sera were evaluated with venereal disease research laboratory (VDRL) and T. pallidum hemagglutination (TPHA) assays. Assuming both methods were confirmatory, our data showed that PCR and serology did not correlate well (agreement = 52.3%, kappa 0.0512, 95% CI -0.0928-0.1951, p = 0.496). The sensitivities, specificities, positive and negative predictive values of the PCR assays were 76.1%, 100%, 100% and 57.9%, respectively, while the values for serology were 62.5%, 100%, 100% and 45.2%, respectively. The combination of PCR and serology can offer valuable information for the diagnosis of syphilis in patients presenting with anogenital ulceration avoiding further clinical complications and disease transmission.
ER  -

NODA, Angel A., Islay RODRIGUEZ, Linda GRILLOVÁ, Philipp P. BOSSHARD a Reto LIENHARD. Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary. \textit{INTERNATIONAL JOURNAL OF STD \&{}amp; AIDS}. LONDON: SAGE PUBLICATIONS LTD, 2019, roč.~30, č.~11, s.~1087-1094. ISSN~0956-4624. Dostupné z: https://dx.doi.org/10.1177/0956462419859764.

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