Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary

NODA, Angel A., Islay RODRIGUEZ, Linda GRILLOVÁ, Philipp P. BOSSHARD a Reto LIENHARD. Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary. INTERNATIONAL JOURNAL OF STD & AIDS. LONDON: SAGE PUBLICATIONS LTD, 2019, roč. 30, č. 11, s. 1087-1094. ISSN 0956-4624. Dostupné z: https://dx.doi.org/10.1177/0956462419859764.

Základní údaje

• Originální název: Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary
• Autoři: NODA, Angel A. (192 Kuba, garant), Islay RODRIGUEZ (192 Kuba), Linda GRILLOVÁ (203 Česká republika, domácí), Philipp P. BOSSHARD (756 Švýcarsko) a Reto LIENHARD (756 Švýcarsko).
• Vydání: INTERNATIONAL JOURNAL OF STD & AIDS, LONDON, SAGE PUBLICATIONS LTD, 2019, 0956-4624.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30303 Infectious Diseases
• Stát vydavatele: Velká Británie a Severní Irsko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 1.406
• Kód RIV: RIV/00216224:14110/19:00112504
• Organizační jednotka: Lékařská fakulta
• Doi: http://dx.doi.org/10.1177/0956462419859764
• UT WoS: 000490799700001
• Klíčová slova anglicky: Syphilis diagnosis; PCR; serology
• Štítky: 14110513, rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid and accurate diagnosis is crucial to ensure patients and their contacts receive timely treatment to eradicate infection and prevent further transmission. In this prospective observational study, we evaluated the performance of polymerase chain reaction (PCR) and serological testing for the diagnosis of primary syphilis by evaluating anogenital swabs and sera from 178 Cuban patients presenting with ulcers. Three different PCR assays were evaluated targeting polA, tpp47 and 16S rDNA loci. Sera were evaluated with venereal disease research laboratory (VDRL) and T. pallidum hemagglutination (TPHA) assays. Assuming both methods were confirmatory, our data showed that PCR and serology did not correlate well (agreement = 52.3%, kappa 0.0512, 95% CI -0.0928-0.1951, p = 0.496). The sensitivities, specificities, positive and negative predictive values of the PCR assays were 76.1%, 100%, 100% and 57.9%, respectively, while the values for serology were 62.5%, 100%, 100% and 45.2%, respectively. The combination of PCR and serology can offer valuable information for the diagnosis of syphilis in patients presenting with anogenital ulceration avoiding further clinical complications and disease transmission.