TESAŘOVÁ, Marie, Filip DUŠA, Marie HORKA and Karel SLAIS. UTILIZATION OF MICROPREPARATIVE FAST FOCUSING BY A NEW WIDE pH RANGE ELECTROLYTE SYSTEM BASED ON BIDIRECTIONAL ISOTACHOFORESIS. CHEMICKÉ LISTY. Praha: Česká společnost chemická, 2013, vol. 107, No 3, p. "S471"-"S472", 2 pp. ISSN 0009-2770.
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Basic information
Original name UTILIZATION OF MICROPREPARATIVE FAST FOCUSING BY A NEW WIDE pH RANGE ELECTROLYTE SYSTEM BASED ON BIDIRECTIONAL ISOTACHOFORESIS
Authors TESAŘOVÁ, Marie, Filip DUŠA, Marie HORKA and Karel SLAIS.
Edition CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2013, 0009-2770.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10400 1.4 Chemical sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.196
Organization unit Faculty of Science
UT WoS 000328730800076
Keywords in English electrolyte system
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 29/7/2020 09:54.
Abstract
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
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