| FAKTOR, Jakub, Lucia KNOPFOVÁ, Petr LAPČÍK, Lucia JANÁČOVÁ, Vendula PÁRALOVÁ, Pavla BOUCHALOVÁ, Petr MÜLLER, Petr BENEŠ a Pavel BOUCHAL. Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis. Proteomics. HOBOKEN, USA: Wiley, 2019, roč. 19, 21-22, s. 1-11. ISSN 1615-9853. Dostupné z: https://dx.doi.org/10.1002/pmic.201900073. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1606911,
author = {Faktor, Jakub and Knopfová, Lucia and Lapčík, Petr and Janáčová, Lucia and Páralová, Vendula and Bouchalová, Pavla and Müller, Petr and Beneš, Petr and Bouchal, Pavel},
article_location = {HOBOKEN, USA},
article_number = {21-22},
doi = {http://dx.doi.org/10.1002/pmic.201900073},
keywords = {breast cancer; mass spectrometry; metastasis; sequential window acquisition of all theoretical; transmembrane proteins},
language = {eng},
issn = {1615-9853},
journal = {Proteomics},
title = {Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis},
url = {http://dx.doi.org/10.1002/pmic.201900073},
volume = {19},
year = {2019}
}
TY - JOUR
ID - 1606911
AU - Faktor, Jakub - Knopfová, Lucia - Lapčík, Petr - Janáčová, Lucia - Páralová, Vendula - Bouchalová, Pavla - Müller, Petr - Beneš, Petr - Bouchal, Pavel
PY - 2019
TI - Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis
JF - Proteomics
VL - 19
IS - 21-22
SP - 1-11
EP - 1-11
PB - Wiley
SN - 16159853
KW - breast cancer
KW - mass spectrometry
KW - metastasis
KW - sequential window acquisition of all theoretical
KW - transmembrane proteins
UR - http://dx.doi.org/10.1002/pmic.201900073
L2 - http://dx.doi.org/10.1002/pmic.201900073
N2 - Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified proteins (FDR < 0.01), 128 differentially abundant cell surface proteins with at least one transmembrane domain are found. Of these, i) desmocollin-1 (DSC1) is validated as a protein connected with lymph node status of luminal A breast cancer, tumor grade, and Her-2 status by immunohistochemistry in the set of 96 primary breast tumors, and ii) catechol-O-methyltransferase is successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as with tumor grade by targeted data extraction from the SWATH-MS data of the same set of tissues. The findings indicate importance of both proteins for breast cancer development and metastasis and highlight the potential of biomarker validation strategy via targeted data extraction from SWATH-MS datasets.
ER -
FAKTOR, Jakub, Lucia KNOPFOVÁ, Petr LAPČÍK, Lucia JANÁČOVÁ, Vendula PÁRALOVÁ, Pavla BOUCHALOVÁ, Petr MÜLLER, Petr BENEŠ a Pavel BOUCHAL. Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis. \textit{Proteomics}. HOBOKEN, USA: Wiley, 2019, roč.~19, 21-22, s.~1-11. ISSN~1615-9853. Dostupné z: https://dx.doi.org/10.1002/pmic.201900073.
|
