Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus
A Strains with G8P[8] Genotype in Human Patients in the Czech
Republic

J 2019

Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ, Jana PRODĚLALOVÁ et. al.

Základní údaje

Originální název

Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic

Autoři

MOUTELÍKOVÁ, Romana (203 Česká republika, garant), Pavel SAUER (203 Česká republika), Monika DVOŘÁKOVÁ HEROLDOVÁ (203 Česká republika, domácí), Veronika HOLÁ (203 Česká republika, domácí) a Jana PRODĚLALOVÁ (203 Česká republika)

Vydání

VIRUSES-BASEL, BASEL, MDPI AG, 2019, 1999-4915

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.816

Kód RIV

RIV/00216224:14110/19:00108575

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/v11111015

UT WoS

000502292300043

Klíčová slova anglicky

rotavirus A; G8; gastroenteritis; bovine-human reassortants; Central Europe

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 10:45, Mgr. Tereza Miškechová

Anotace

V originále

Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, but interspecies transmission and reassortment between human and animal RVAs significantly contribute to their genetic diversity. We investigated the VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). Sequence analysis of G8P[8] strains revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains that were isolated in Vietnam in 2014/2015. The whole-genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. Phylogenetic analysis of all segments clustered the Czech isolates with RVA strains that were formerly described in Southeast Asia, which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS-1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.

Návaznosti

NV16-29937A, projekt VaV

Název: Komplexní analýza humánních rotavirových infekcí v České republice včetně atypických a emergentních kmenů směřující k vývoji nových detekčních metod

Citovat

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ a Jana PRODĚLALOVÁ. Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic. VIRUSES-BASEL. BASEL: MDPI AG, 2019, roč. 11, č. 11, s. 1-14. ISSN 1999-4915. Dostupné z: https://dx.doi.org/10.3390/v11111015.

@article{1607099,
   author = {Moutelíková, Romana and Sauer, Pavel and Dvořáková Heroldová, Monika and Holá, Veronika and Prodělalová, Jana},
   article_location = {BASEL},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/v11111015},
   keywords = {rotavirus A; G8; gastroenteritis; bovine-human reassortants; Central Europe},
   language = {eng},
   issn = {1999-4915},
   journal = {VIRUSES-BASEL},
   title = {Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic},
   url = {http://dx.doi.org/10.3390/v11111015},
   volume = {11},
   year = {2019}
}

TY  - JOUR
ID  - 1607099
AU  - Moutelíková, Romana - Sauer, Pavel - Dvořáková Heroldová, Monika - Holá, Veronika - Prodělalová, Jana
PY  - 2019
TI  - Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic
JF  - VIRUSES-BASEL
VL  - 11
IS  - 11
SP  - 1-14
EP  - 1-14
PB  - MDPI AG
SN  - 19994915
KW  - rotavirus A
KW  - G8
KW  - gastroenteritis
KW  - bovine-human reassortants
KW  - Central Europe
UR  - http://dx.doi.org/10.3390/v11111015
L2  - http://dx.doi.org/10.3390/v11111015
N2  - Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, but interspecies transmission and reassortment between human and animal RVAs significantly contribute to their genetic diversity. We investigated the VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). Sequence analysis of G8P[8] strains revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains that were isolated in Vietnam in 2014/2015. The whole-genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. Phylogenetic analysis of all segments clustered the Czech isolates with RVA strains that were formerly described in Southeast Asia, which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS-1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.
ER  -

MOUTELÍKOVÁ, Romana, Pavel SAUER, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ a Jana PRODĚLALOVÁ. Emergence of Rare Bovine-Human Reassortant DS-1-Like Rotavirus A Strains with G8P[8] Genotype in Human Patients in the Czech Republic. \textit{VIRUSES-BASEL}. BASEL: MDPI AG, 2019, roč.~11, č.~11, s.~1-14. ISSN~1999-4915. Dostupné z: https://dx.doi.org/10.3390/v11111015.

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