Tuftelin and HIFs expression in osteogenesis

J 2019

Tuftelin and HIFs expression in osteogenesis

BOBEK, Jan, Veronika ORALOVÁ, Adéla KRATOCHVÍLOVÁ, Ivana ŽVÁČKOVÁ, H LESOT et. al.

Základní údaje

Originální název

Tuftelin and HIFs expression in osteogenesis

Autoři

BOBEK, Jan, Veronika ORALOVÁ, Adéla KRATOCHVÍLOVÁ, Ivana ŽVÁČKOVÁ, H LESOT a Eva MATALOVÁ

Vydání

Histochemistry and Cell Biology, Heidelberg, Springer, 2019, 0948-6143

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.418

DOI

http://dx.doi.org/10.1007/s00418-019-01813-4

UT WoS

000495293400005

Klíčová slova anglicky

Intramembranous; Ossification; Bone; Tuftelin; HIF1; HIF2

Štítky

RIV ne

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 1. 2020 09:35, Mgr. Adéla Kratochvílová, Ph.D.

Anotace

V originále

Tuftelin was originally discovered and mostly studied in the tooth, but later found also in other organs. Despite its wide distribution among tissues, tuftelin's function has so far been specified only in the formation of enamel crystals. Nevertheless, in many cases, tuftelin was suggested to be associated with cellular adaptation to hypoxia and recently even with cell differentiation. Therefore, we aimed to investigate tuftelin expression along with hypoxia-inducible factors (HIFs) during the early development of the mandibular/alveolar (m/a) bone, when osteoblasts started to differentiate in vivo and to compare their expression levels in undifferentiated versus differentiated osteoblastic cells in vitro. Immunohistochemistry demonstrated the presence of tuftelin already in osteoblastic precursors which were also HIF1-positive, but HIF2-negative. Nevertheless, HIF2 protein appeared when osteoblasts differentiated, one day later. This is in agreement with observations made with MC3T3-E1 cells, where there was no significant difference in tuftelin and Hif1 expression in undifferentiated vs. differentiated cells, although Hif2 increased upon differentiation induction. In differentiated osteoblasts of the m/a bone, all three proteins accumulated, first, prenatally, in the cytoplasm and later, particularly at postnatal stages, they displayed also peri/nuclear localization. Such a dynamic time-space pattern of tuftelin expression has recently been reported in neurons, which, as the m/a bone, differentiate under less hypoxic conditions as indicated also by a prevalent cytoplasmic expression of HIF1 in osteoblasts. However, unlike what was shown in cultured neurons, tuftelin does not seem to participate in final osteoblastic differentiation and its functions, thus, appears to be tissue specific.

Citovat

BOBEK, Jan, Veronika ORALOVÁ, Adéla KRATOCHVÍLOVÁ, Ivana ŽVÁČKOVÁ, H LESOT a Eva MATALOVÁ. Tuftelin and HIFs expression in osteogenesis. Histochemistry and Cell Biology. Heidelberg: Springer, 2019, roč. 152, č. 5, s. 355-363. ISSN 0948-6143. Dostupné z: https://dx.doi.org/10.1007/s00418-019-01813-4.

@article{1607529,
   author = {Bobek, Jan and Oralová, Veronika and Kratochvílová, Adéla and Žváčková, Ivana and Lesot, H and Matalová, Eva},
   article_location = {Heidelberg},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00418-019-01813-4},
   keywords = {Intramembranous; Ossification; Bone; Tuftelin; HIF1; HIF2},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Tuftelin and HIFs expression in osteogenesis},
   url = {http://dx.doi.org/10.1007/s00418-019-01813-4},
   volume = {152},
   year = {2019}
}

TY  - JOUR
ID  - 1607529
AU  - Bobek, Jan - Oralová, Veronika - Kratochvílová, Adéla - Žváčková, Ivana - Lesot, H - Matalová, Eva
PY  - 2019
TI  - Tuftelin and HIFs expression in osteogenesis
JF  - Histochemistry and Cell Biology
VL  - 152
IS  - 5
SP  - 355-363
EP  - 355-363
PB  - Springer
SN  - 09486143
KW  - Intramembranous
KW  - Ossification
KW  - Bone
KW  - Tuftelin
KW  - HIF1
KW  - HIF2
UR  - http://dx.doi.org/10.1007/s00418-019-01813-4
L2  - http://dx.doi.org/10.1007/s00418-019-01813-4
N2  - Tuftelin was originally discovered and mostly studied in the tooth, but later found also in other organs. Despite its wide distribution among tissues, tuftelin's function has so far been specified only in the formation of enamel crystals. Nevertheless, in many cases, tuftelin was suggested to be associated with cellular adaptation to hypoxia and recently even with cell differentiation. Therefore, we aimed to investigate tuftelin expression along with hypoxia-inducible factors (HIFs) during the early development of the mandibular/alveolar (m/a) bone, when osteoblasts started to differentiate in vivo and to compare their expression levels in undifferentiated versus differentiated osteoblastic cells in vitro. Immunohistochemistry demonstrated the presence of tuftelin already in osteoblastic precursors which were also HIF1-positive, but HIF2-negative. Nevertheless, HIF2 protein appeared when osteoblasts differentiated, one day later. This is in agreement with observations made with MC3T3-E1 cells, where there was no significant difference in tuftelin and Hif1 expression in undifferentiated vs. differentiated cells, although Hif2 increased upon differentiation induction. In differentiated osteoblasts of the m/a bone, all three proteins accumulated, first, prenatally, in the cytoplasm and later, particularly at postnatal stages, they displayed also peri/nuclear localization. Such a dynamic time-space pattern of tuftelin expression has recently been reported in neurons, which, as the m/a bone, differentiate under less hypoxic conditions as indicated also by a prevalent cytoplasmic expression of HIF1 in osteoblasts. However, unlike what was shown in cultured neurons, tuftelin does not seem to participate in final osteoblastic differentiation and its functions, thus, appears to be tissue specific.
ER  -

BOBEK, Jan, Veronika ORALOVÁ, Adéla KRATOCHVÍLOVÁ, Ivana ŽVÁČKOVÁ, H LESOT a Eva MATALOVÁ. Tuftelin and HIFs expression in osteogenesis. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2019, roč.~152, č.~5, s.~355-363. ISSN~0948-6143. Dostupné z: https://dx.doi.org/10.1007/s00418-019-01813-4.

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