| STARA, Jitka, Stanislav PEKÁR, Marta NESVORNA, Tomas ERBAN, Hana VINSOVA, Jan KOPECKY, Ivo DOSKOCIL, Martin KAMLER a Jan HUBERT. Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene. Experimental and Applied Acarology. DORDRECHT: SPRINGER, 2019, roč. 77, č. 2, s. 161-171. ISSN 0168-8162. Dostupné z: https://dx.doi.org/10.1007/s10493-019-00353-9. |
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@article{1607587,
author = {Stara, Jitka and Pekár, Stanislav and Nesvorna, Marta and Erban, Tomas and Vinsova, Hana and Kopecky, Jan and Doskocil, Ivo and Kamler, Martin and Hubert, Jan},
article_location = {DORDRECHT},
article_number = {2},
doi = {http://dx.doi.org/10.1007/s10493-019-00353-9},
keywords = {Varroa destructor; Honey bee; Apiary; Acaricide; Restriction},
language = {eng},
issn = {0168-8162},
journal = {Experimental and Applied Acarology},
title = {Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene},
url = {https://link.springer.com/article/10.1007%2Fs10493-019-00353-9},
volume = {77},
year = {2019}
}
TY - JOUR
ID - 1607587
AU - Stara, Jitka - Pekár, Stanislav - Nesvorna, Marta - Erban, Tomas - Vinsova, Hana - Kopecky, Jan - Doskocil, Ivo - Kamler, Martin - Hubert, Jan
PY - 2019
TI - Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene
JF - Experimental and Applied Acarology
VL - 77
IS - 2
SP - 161-171
EP - 161-171
PB - SPRINGER
SN - 01688162
KW - Varroa destructor
KW - Honey bee
KW - Apiary
KW - Acaricide
KW - Restriction
UR - https://link.springer.com/article/10.1007%2Fs10493-019-00353-9
L2 - https://link.springer.com/article/10.1007%2Fs10493-019-00353-9
N2 - In this study, we used the discriminating concentration of tau-fluvalinate (0.25 mu g/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (Na(V)Ch), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites.
ER -
STARA, Jitka, Stanislav PEKÁR, Marta NESVORNA, Tomas ERBAN, Hana VINSOVA, Jan KOPECKY, Ivo DOSKOCIL, Martin KAMLER a Jan HUBERT. Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene. \textit{Experimental and Applied Acarology}. DORDRECHT: SPRINGER, 2019, roč.~77, č.~2, s.~161-171. ISSN~0168-8162. Dostupné z: https://dx.doi.org/10.1007/s10493-019-00353-9.
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