STARA, Jitka, Stanislav PEKÁR, Marta NESVORNA, Tomas ERBAN, Hana VINSOVA, Jan KOPECKY, Ivo DOSKOCIL, Martin KAMLER a Jan HUBERT. Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene. Experimental and Applied Acarology. DORDRECHT: SPRINGER, 2019, roč. 77, č. 2, s. 161-171. ISSN 0168-8162. Dostupné z: https://dx.doi.org/10.1007/s10493-019-00353-9. |
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@article{1607587, author = {Stara, Jitka and Pekár, Stanislav and Nesvorna, Marta and Erban, Tomas and Vinsova, Hana and Kopecky, Jan and Doskocil, Ivo and Kamler, Martin and Hubert, Jan}, article_location = {DORDRECHT}, article_number = {2}, doi = {http://dx.doi.org/10.1007/s10493-019-00353-9}, keywords = {Varroa destructor; Honey bee; Apiary; Acaricide; Restriction}, language = {eng}, issn = {0168-8162}, journal = {Experimental and Applied Acarology}, title = {Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene}, url = {https://link.springer.com/article/10.1007%2Fs10493-019-00353-9}, volume = {77}, year = {2019} }
TY - JOUR ID - 1607587 AU - Stara, Jitka - Pekár, Stanislav - Nesvorna, Marta - Erban, Tomas - Vinsova, Hana - Kopecky, Jan - Doskocil, Ivo - Kamler, Martin - Hubert, Jan PY - 2019 TI - Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene JF - Experimental and Applied Acarology VL - 77 IS - 2 SP - 161-171 EP - 161-171 PB - SPRINGER SN - 01688162 KW - Varroa destructor KW - Honey bee KW - Apiary KW - Acaricide KW - Restriction UR - https://link.springer.com/article/10.1007%2Fs10493-019-00353-9 L2 - https://link.springer.com/article/10.1007%2Fs10493-019-00353-9 N2 - In this study, we used the discriminating concentration of tau-fluvalinate (0.25 mu g/mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (Na(V)Ch), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites. ER -
STARA, Jitka, Stanislav PEKÁR, Marta NESVORNA, Tomas ERBAN, Hana VINSOVA, Jan KOPECKY, Ivo DOSKOCIL, Martin KAMLER a Jan HUBERT. Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene. \textit{Experimental and Applied Acarology}. DORDRECHT: SPRINGER, 2019, roč.~77, č.~2, s.~161-171. ISSN~0168-8162. Dostupné z: https://dx.doi.org/10.1007/s10493-019-00353-9.
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