Calcium signaling affects migration and proliferation
differently in individual cancer cells due to nifedipine
treatment

J 2020

Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment

CHOVANCOVÁ, Barbora, Veronika LISKOVÁ, Svetlana MIKLIKOVA, Sona HUDECOVA, Petr BABULA et. al.

Základní údaje

Originální název

Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment

Autoři

CHOVANCOVÁ, Barbora (703 Slovensko), Veronika LISKOVÁ (703 Slovensko), Svetlana MIKLIKOVA (703 Slovensko), Sona HUDECOVA (703 Slovensko), Petr BABULA (203 Česká republika, domácí), Adela PENESOVA (703 Slovensko), Angelika SEVCIKOVA (703 Slovensko), Erika DURINIKOVA (703 Slovensko), Marie NOVÁKOVÁ (203 Česká republika, domácí), Miroslava MATUSKOVA (703 Slovensko) a Oľga KRIŽANOVÁ (703 Slovensko, garant, domácí)

Vydání

Biochemical Pharmacology, Oxford, Elsevier, 2020, 0006-2952

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.858

Kód RIV

RIV/00216224:14110/20:00115241

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.bcp.2019.113695

UT WoS

000519219000014

Klíčová slova anglicky

Breast cancer; Sodium calcium exchanger 1; Inositol 1_4_5-trisphosphate receptor; Apoptosis Migration

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 7. 2020 09:47, Mgr. Tereza Miškechová

Anotace

V originále

Several papers have reported that calcium channel blocking drugs were associated with increased breast cancer risk and worsened prognosis. One of the most common signs of breast tumors is the presence of small deposits of calcium, known as microcalcifications. Therefore, we studied the effect of dihydropyridine nifedipine on selected calcium transport systems in MDA-MB-231 cells, originating from triple negative breast tumor and JIMT1 cells that represent a model of HER2-positive breast cancer, which possesses amplification of HER2 receptor, but cells do not response to HER2 inhibition treatment with trastuzumab. Also, we compared the effect of nifedipine on colorectal DLD1 and ovarian A2780 cancer cells. Both, inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) and type 1 sodium calcium exchanger (NCX1) were upregulated due to nifedipine in DLD1 and A2780 cells, but not in breast cancer MDA-MB-231 and JIMT1 cells. On contrary to MDA-MB-231 and JIMT1 cells, in DLD1 and A2780 cells nifedipine induced apoptosis in a concentration-dependent manner. After NCX1 silencing and subsequent treatment with nifedipine, proliferation was decreased in MDA-MB-231, increased in DLD1 cells, and not changed in JIMT1 cells. Silencing of IP3R1 revealed increase in proliferation in DLD1 and JIMT1 cells, but caused decrease in proliferation in MDA-MB-231 cell line after nifedipine treatment. Interestingly, after nifedipine treatment migration was not significantly affected in any of tested cell lines after NCX1 silencing. Due to IP3R1 silencing, significant decrease in migration occurred in MDA-MB-231 cells after nifedipine treatment, but not in other tested cells. These results support different function of the NCX1 and IP3R1 in the invasiveness of various cancer cells due to nifedipine treatment.

Návaznosti

MUNI/A/1255/2018, interní kód MU

Název: Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak (Akronym: KAREDYSFAR)

Investor: Masarykova univerzita, Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

CHOVANCOVÁ, Barbora, Veronika LISKOVÁ, Svetlana MIKLIKOVA, Sona HUDECOVA, Petr BABULA, Adela PENESOVA, Angelika SEVCIKOVA, Erika DURINIKOVA, Marie NOVÁKOVÁ, Miroslava MATUSKOVA a Oľga KRIŽANOVÁ. Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment. Biochemical Pharmacology. Oxford: Elsevier, 2020, roč. 171, January 2020, s. 1-15. ISSN 0006-2952. Dostupné z: https://dx.doi.org/10.1016/j.bcp.2019.113695.

@article{1609497,
   author = {Chovancová, Barbora and Lisková, Veronika and Miklikova, Svetlana and Hudecova, Sona and Babula, Petr and Penesova, Adela and Sevcikova, Angelika and Durinikova, Erika and Nováková, Marie and Matuskova, Miroslava and Križanová, Oľga},
   article_location = {Oxford},
   article_number = {January 2020},
   doi = {http://dx.doi.org/10.1016/j.bcp.2019.113695},
   keywords = {Breast cancer; Sodium calcium exchanger 1; Inositol 1_4_5-trisphosphate receptor; Apoptosis Migration},
   language = {eng},
   issn = {0006-2952},
   journal = {Biochemical Pharmacology},
   title = {Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment},
   url = {https://www.sciencedirect.com/science/article/pii/S0006295219303946?via%3Dihub},
   volume = {171},
   year = {2020}
}

TY  - JOUR
ID  - 1609497
AU  - Chovancová, Barbora - Lisková, Veronika - Miklikova, Svetlana - Hudecova, Sona - Babula, Petr - Penesova, Adela - Sevcikova, Angelika - Durinikova, Erika - Nováková, Marie - Matuskova, Miroslava - Križanová, Oľga
PY  - 2020
TI  - Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment
JF  - Biochemical Pharmacology
VL  - 171
IS  - January 2020
SP  - 1-15
EP  - 1-15
PB  - Elsevier
SN  - 00062952
KW  - Breast cancer
KW  - Sodium calcium exchanger 1
KW  - Inositol 1_4_5-trisphosphate receptor
KW  - Apoptosis Migration
UR  - https://www.sciencedirect.com/science/article/pii/S0006295219303946?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0006295219303946?via%3Dihub
N2  - Several papers have reported that calcium channel blocking drugs were associated with increased breast cancer risk and worsened prognosis. One of the most common signs of breast tumors is the presence of small deposits of calcium, known as microcalcifications. Therefore, we studied the effect of dihydropyridine nifedipine on selected calcium transport systems in MDA-MB-231 cells, originating from triple negative breast tumor and JIMT1 cells that represent a model of HER2-positive breast cancer, which possesses amplification of HER2 receptor, but cells do not response to HER2 inhibition treatment with trastuzumab. Also, we compared the effect of nifedipine on colorectal DLD1 and ovarian A2780 cancer cells. Both, inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) and type 1 sodium calcium exchanger (NCX1) were upregulated due to nifedipine in DLD1 and A2780 cells, but not in breast cancer MDA-MB-231 and JIMT1 cells. On contrary to MDA-MB-231 and JIMT1 cells, in DLD1 and A2780 cells nifedipine induced apoptosis in a concentration-dependent manner. After NCX1 silencing and subsequent treatment with nifedipine, proliferation was decreased in MDA-MB-231, increased in DLD1 cells, and not changed in JIMT1 cells. Silencing of IP3R1 revealed increase in proliferation in DLD1 and JIMT1 cells, but caused decrease in proliferation in MDA-MB-231 cell line after nifedipine treatment. Interestingly, after nifedipine treatment migration was not significantly affected in any of tested cell lines after NCX1 silencing. Due to IP3R1 silencing, significant decrease in migration occurred in MDA-MB-231 cells after nifedipine treatment, but not in other tested cells. These results support different function of the NCX1 and IP3R1 in the invasiveness of various cancer cells due to nifedipine treatment.
ER  -

CHOVANCOVÁ, Barbora, Veronika LISKOVÁ, Svetlana MIKLIKOVA, Sona HUDECOVA, Petr BABULA, Adela PENESOVA, Angelika SEVCIKOVA, Erika DURINIKOVA, Marie NOVÁKOVÁ, Miroslava MATUSKOVA a Oľga KRIŽANOVÁ. Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment. \textit{Biochemical Pharmacology}. Oxford: Elsevier, 2020, roč.~171, January 2020, s.~1-15. ISSN~0006-2952. Dostupné z: https://dx.doi.org/10.1016/j.bcp.2019.113695.

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