Analysis of bicarbonate, phosphate and other anions in saliva
by capillary electrophoresis with capacitively coupled
contactless conductivity detection in diagnostics of
gastroesophageal reflux disease

J 2020

Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET et. al.

Základní údaje

Originální název

Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease

Autoři

DOSEDĚLOVÁ, Věra (203 Česká republika, domácí), Pavol ĎURČ (703 Slovensko, domácí), Jiří DOLINA (203 Česká republika, domácí), Štefan KONEČNÝ (703 Slovensko, domácí), František FORET (203 Česká republika, domácí) a Petr KUBÁŇ (203 Česká republika, garant, domácí)

Vydání

Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2020, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.535

Kód RIV

RIV/00216224:14740/20:00115254

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/elps.201900319

UT WoS

000495131500001

Klíčová slova anglicky

Bicarbonate; Capillary electrophoresis; Gastroesophageal reflux disease; Phosphate; Saliva

Štítky

14110213, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2021 20:38, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 mu M cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-(CD)-D-4.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV17-31945A, projekt VaV

Název: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET a Petr KUBÁŇ. Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2020, roč. 41, 1-2, s. 116-122. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900319.

@article{1611436,
   author = {Dosedělová, Věra and Ďurč, Pavol and Dolina, Jiří and Konečný, Štefan and Foret, František and Kubáň, Petr},
   article_location = {HOBOKEN},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1002/elps.201900319},
   keywords = {Bicarbonate; Capillary electrophoresis; Gastroesophageal reflux disease; Phosphate; Saliva},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900319},
   volume = {41},
   year = {2020}
}

TY  - JOUR
ID  - 1611436
AU  - Dosedělová, Věra - Ďurč, Pavol - Dolina, Jiří - Konečný, Štefan - Foret, František - Kubáň, Petr
PY  - 2020
TI  - Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease
JF  - Electrophoresis
VL  - 41
IS  - 1-2
SP  - 116-122
EP  - 116-122
PB  - WILEY-BLACKWELL
SN  - 01730835
KW  - Bicarbonate
KW  - Capillary electrophoresis
KW  - Gastroesophageal reflux disease
KW  - Phosphate
KW  - Saliva
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900319
N2  - Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 mu M cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-(CD)-D-4.
ER  -

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET a Petr KUBÁŇ. Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2020, roč.~41, 1-2, s.~116-122. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900319.

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