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@article{1612117,
author = {Ashrafi, A.M. and Sys, M. and Sedlackova, E. and Farag, A.S. and Adam, V. and Přibyl, Jan and Richtera, L.},
article_location = {Basel},
article_number = {13},
doi = {http://dx.doi.org/10.3390/s19132939},
keywords = {glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals},
url = {https://www.mdpi.com/1424-8220/19/13/2939},
volume = {19},
year = {2019}
}
TY - JOUR
ID - 1612117
AU - Ashrafi, A.M. - Sys, M. - Sedlackova, E. - Farag, A.S. - Adam, V. - Přibyl, Jan - Richtera, L.
PY - 2019
TI - Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
JF - Sensors
VL - 19
IS - 13
SP - 2939
EP - 2939
PB - MDPI AG
SN - 14248220
KW - glucose oxidase
KW - heavy metals
KW - amperometric biosensor
KW - non-competitive inhibition
UR - https://www.mdpi.com/1424-8220/19/13/2939
L2 - https://www.mdpi.com/1424-8220/19/13/2939
N2 - The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.
ER -
ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM, Jan PŘIBYL a L. RICHTERA. Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals. \textit{Sensors}. Basel: MDPI AG, 2019, roč.~19, č.~13, s.~2939-2952. ISSN~1424-8220. Dostupné z: https://dx.doi.org/10.3390/s19132939.
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