Biphasic calcium phosphate scaffolds with controlled pore size
distribution prepared by in-situ foaming

J 2019

Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR et. al.

Základní údaje

Originální název

Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming

Autoři

NOVOTNA, Lenka (203 Česká republika, garant), Lukáš KUČERA (203 Česká republika, domácí), Aleš HAMPL (203 Česká republika, domácí), Daniel DRDLIK (203 Česká republika), Jaroslav CIHLAR (203 Česká republika) a Jaroslav CIHLAR (203 Česká republika)

Vydání

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, AMSTERDAM, ELSEVIER SCIENCE BV, 2019, 0928-4931

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20902 Bioprocessing technologies biocatalysis, fermentation

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.880

Kód RIV

RIV/00216224:14110/19:00112788

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.msec.2018.03.022

UT WoS

000455858300039

Klíčová slova anglicky

Calcium phosphate; Scaffold; Porosity; Bioactivity; Biocompatibility

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2020 09:54, Mgr. Tereza Miškechová

Anotace

V originále

In this study, a reproducible method of fabricating hierarchically 3D porous scaffolds with high porosity and pore interconnectivity is reported. The method is based on in-situ foaming of a dispersion of diisocyanate, polyol, water and hydroxyapatite (HA) to form a hard foamed HA/polyurethane composite which after heat treatment provided a bi-phase calcium phosphate scaffold. This technique, combining the advantages of polymer sponge and direct foaming methods, provides a better control over the macrostructure of the scaffold. A modification of the multi-scaled porous macrostructure of scaffolds produced by changing the ratio of input reactants and by sintering temperature was studied. The pore morphology, size, and distribution were characterized using a scanning electron microscope and mercury porosimetry. The pores were open and interconnected with multi-scale (from several nanometres to millimetres) sizes convenient for using in tissue engineering applications. The bioactivity was confirmed by growing an apatite layer on the surfaces after immersion in simulated body fluid. The material was biocompatible, as shown by using normal human adipose tissue-derived stem cells (ASC). When seeded onto the scaffolds, the ASC adhered and remained healthy while maintaining their typical morphology.

Návaznosti

LD15144, projekt VaV

Název: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Akronym: TissueENG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Buněčné a nebuněčné základy pro regeneraci kostí a zubů

LM2015041, projekt VaV

Název: CEITEC Nano

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC Nano

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR a Jaroslav CIHLAR. Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, roč. 95, FEB 1 2019, s. 363-370. ISSN 0928-4931. Dostupné z: https://dx.doi.org/10.1016/j.msec.2018.03.022.

@article{1612166,
   author = {Novotna, Lenka and Kučera, Lukáš and Hampl, Aleš and Drdlik, Daniel and Cihlar, Jaroslav and Cihlar, Jaroslav},
   article_location = {AMSTERDAM},
   article_number = {FEB 1 2019},
   doi = {http://dx.doi.org/10.1016/j.msec.2018.03.022},
   keywords = {Calcium phosphate; Scaffold; Porosity; Bioactivity; Biocompatibility},
   language = {eng},
   issn = {0928-4931},
   journal = {MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS},
   title = {Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming},
   url = {http://dx.doi.org/10.1016/j.msec.2018.03.022},
   volume = {95},
   year = {2019}
}

TY  - JOUR
ID  - 1612166
AU  - Novotna, Lenka - Kučera, Lukáš - Hampl, Aleš - Drdlik, Daniel - Cihlar, Jaroslav - Cihlar, Jaroslav
PY  - 2019
TI  - Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming
JF  - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
VL  - 95
IS  - FEB 1 2019
SP  - 363-370
EP  - 363-370
PB  - ELSEVIER SCIENCE BV
SN  - 09284931
KW  - Calcium phosphate
KW  - Scaffold
KW  - Porosity
KW  - Bioactivity
KW  - Biocompatibility
UR  - http://dx.doi.org/10.1016/j.msec.2018.03.022
L2  - http://dx.doi.org/10.1016/j.msec.2018.03.022
N2  - In this study, a reproducible method of fabricating hierarchically 3D porous scaffolds with high porosity and pore interconnectivity is reported. The method is based on in-situ foaming of a dispersion of diisocyanate, polyol, water and hydroxyapatite (HA) to form a hard foamed HA/polyurethane composite which after heat treatment provided a bi-phase calcium phosphate scaffold. This technique, combining the advantages of polymer sponge and direct foaming methods, provides a better control over the macrostructure of the scaffold. A modification of the multi-scaled porous macrostructure of scaffolds produced by changing the ratio of input reactants and by sintering temperature was studied. The pore morphology, size, and distribution were characterized using a scanning electron microscope and mercury porosimetry. The pores were open and interconnected with multi-scale (from several nanometres to millimetres) sizes convenient for using in tissue engineering applications. The bioactivity was confirmed by growing an apatite layer on the surfaces after immersion in simulated body fluid. The material was biocompatible, as shown by using normal human adipose tissue-derived stem cells (ASC). When seeded onto the scaffolds, the ASC adhered and remained healthy while maintaining their typical morphology.
ER  -

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR a Jaroslav CIHLAR. Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming. \textit{MATERIALS SCIENCE \&{}amp; ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, roč.~95, FEB 1 2019, s.~363-370. ISSN~0928-4931. Dostupné z: https://dx.doi.org/10.1016/j.msec.2018.03.022.

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