The Influence of Quadruplex Structure in Proximity to P53
Target Sequences on the Transactivation Potential of P53 Alpha
Isoforms

J 2020

The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL et. al.

Základní údaje

Originální název

The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

Autoři

PORUBIAKOVÁ, Otília (703 Slovensko), Natália BOHÁLOVÁ (703 Slovensko, domácí), Alberto INGA (380 Itálie), Natália VADOVIČOVÁ (203 Česká republika, domácí), Jan COUFAL (203 Česká republika), Miroslav FOJTA (203 Česká republika) a Václav BRÁZDA (203 Česká republika, garant)

Vydání

International Journal of Molecular Sciences, Basel, Molecular Diversity Preservation International, 2020, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.923

Kód RIV

RIV/00216224:14310/20:00115260

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/ijms21010127

UT WoS

000515378000127

Klíčová slova anglicky

p53 protein; protein-DNA interaction; transactivation potential

Štítky

14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 8. 2021 16:32, Mgr. Marie Šípková, DiS.

Anotace

V originále

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.

Citovat

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL, Miroslav FOJTA a Václav BRÁZDA. The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms. International Journal of Molecular Sciences. Basel: Molecular Diversity Preservation International, 2020, roč. 21, č. 1, s. 1-12. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21010127.

@article{1612996,
   author = {Porubiaková, Otília and Bohálová, Natália and Inga, Alberto and Vadovičová, Natália and Coufal, Jan and Fojta, Miroslav and Brázda, Václav},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms21010127},
   keywords = {p53 protein; protein-DNA interaction; transactivation potential},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms},
   url = {https://www.mdpi.com/1422-0067/21/1/127},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1612996
AU  - Porubiaková, Otília - Bohálová, Natália - Inga, Alberto - Vadovičová, Natália - Coufal, Jan - Fojta, Miroslav - Brázda, Václav
PY  - 2020
TI  - The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Molecular Diversity Preservation International
SN  - 14220067
KW  - p53 protein
KW  - protein-DNA interaction
KW  - transactivation potential
UR  - https://www.mdpi.com/1422-0067/21/1/127
L2  - https://www.mdpi.com/1422-0067/21/1/127
N2  - p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.
ER  -

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL, Miroslav FOJTA a Václav BRÁZDA. The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms. \textit{International Journal of Molecular Sciences}. Basel: Molecular Diversity Preservation International, 2020, roč.~21, č.~1, s.~1-12. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21010127.

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