Bilateral activation of glial cells and cellular distribution
of the chemokine CCL2 and its receptor CCR2 in the trigeminal
subnucleus caudalis of trigeminal neuropathic pain model

J 2020

Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model

Autoři

KUBÍČKOVÁ, Lucie (203 Česká republika, domácí), Ilona KLUSÁKOVÁ (203 Česká republika, domácí) a Petr DUBOVÝ (203 Česká republika, garant, domácí)

Vydání

Histochemistry and Cell Biology, New York, Springer, 2020, 0948-6143

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.304

Kód RIV

RIV/00216224:14110/20:00115261

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s00418-020-01850-4

UT WoS

000511060400001

Klíčová slova anglicky

unilateral nerve injury; infraorbital nerve; microglia; astrocytes

Štítky

14110514, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 1. 2021 14:08, Mgr. Tereza Miškechová

Anotace

V originále

Glial cells activated by peripheral nerve injury contribute to the induction and maintenance of neuropathic pain by releasing neuromodulating cytokines and chemokines. We investigated the activation of microglia and astrocytes as well as the cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis (TSC) ipsilateral and contralateral to infraorbital nerve ligature (IONL). The left infraorbital nerve was ligated under aseptic conditions, and sham controls were operated without nerve ligature. Tactile hypersensitivity was significantly increased bilaterally in vibrissal pads of both sham- and IONL-operated animals from day 1 to 7 and tended to normalize in sham controls surviving for 14 days. Activated microglial cells significantly increased bilaterally in the TSC of both sham- and IONL-operated animals with a marked but gradual increase in the ipsilateral TSC from 1 to 7 days followed by a decrease by day 14. In contrast, robust activation of astrocytes was found bilaterally in the TSC of IONL-operated rats from 3 to 14 days with a transient activation in the ipsilateral TSC of sham-operated animals. Cellular distribution of CCL2 varied with survival time. CCL2 immunofluorescence was detected in neurons within 3 days and in astrocytes at later time points. In contrast, CCR2 was found only in astrocytes at all time points with CCR2 intensity being dominant in the ipsilateral TSC. In summary, our results reveal bilateral activation of microglial cells and astrocytes as well as changes in the cellular distribution of CCL2 and its receptor CCR2 in the TSC during the development and maintenance of orofacial neuropathic pain.

Návaznosti

MUNI/A/1086/2018, interní kód MU

Název: Reakce struktur nervové soustavy na poškození

Investor: Masarykova univerzita, Reakce struktur nervové soustavy na poškození, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model. Histochemistry and Cell Biology. New York: Springer, 2020, roč. 153, č. 4, s. 239-255. ISSN 0948-6143. Dostupné z: https://dx.doi.org/10.1007/s00418-020-01850-4.

@article{1613460,
   author = {Kubíčková, Lucie and Klusáková, Ilona and Dubový, Petr},
   article_location = {New York},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s00418-020-01850-4},
   keywords = {unilateral nerve injury; infraorbital nerve; microglia; astrocytes},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model},
   url = {https://link.springer.com/article/10.1007/s00418-020-01850-4},
   volume = {153},
   year = {2020}
}

TY  - JOUR
ID  - 1613460
AU  - Kubíčková, Lucie - Klusáková, Ilona - Dubový, Petr
PY  - 2020
TI  - Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model
JF  - Histochemistry and Cell Biology
VL  - 153
IS  - 4
SP  - 239-255
EP  - 239-255
PB  - Springer
SN  - 09486143
KW  - unilateral nerve injury
KW  - infraorbital nerve
KW  - microglia
KW  - astrocytes
UR  - https://link.springer.com/article/10.1007/s00418-020-01850-4
L2  - https://link.springer.com/article/10.1007/s00418-020-01850-4
N2  - Glial cells activated by peripheral nerve injury contribute to the induction and maintenance of neuropathic pain by releasing neuromodulating cytokines and chemokines. We investigated the activation of microglia and astrocytes as well as the cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis (TSC) ipsilateral and contralateral to infraorbital nerve ligature (IONL). The left infraorbital nerve was ligated under aseptic conditions, and sham controls were operated without nerve ligature. Tactile hypersensitivity was significantly increased bilaterally in vibrissal pads of both sham- and IONL-operated animals from day 1 to 7 and tended to normalize in sham controls surviving for 14 days. Activated microglial cells significantly increased bilaterally in the TSC of both sham- and IONL-operated animals with a marked but gradual increase in the ipsilateral TSC from 1 to 7 days followed by a decrease by day 14. In contrast, robust activation of astrocytes was found bilaterally in the TSC of IONL-operated rats from 3 to 14 days with a transient activation in the ipsilateral TSC of sham-operated animals. Cellular distribution of CCL2 varied with survival time. CCL2 immunofluorescence was detected in neurons within 3 days and in astrocytes at later time points. In contrast, CCR2 was found only in astrocytes at all time points with CCR2 intensity being dominant in the ipsilateral TSC. In summary, our results reveal bilateral activation of microglial cells and astrocytes as well as changes in the cellular distribution of CCL2 and its receptor CCR2 in the TSC during the development and maintenance of orofacial neuropathic pain.
ER  -

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model. \textit{Histochemistry and Cell Biology}. New York: Springer, 2020, roč.~153, č.~4, s.~239-255. ISSN~0948-6143. Dostupné z: https://dx.doi.org/10.1007/s00418-020-01850-4.

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