Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

BRAZERT, Maciej, Wieslawa KRANC, Blazej CHERMULA, Katarzyna KOWALSKA, Maurycy JANKOWSKI, Piotr CELICHOWSKI, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Leszek PAWELCZYK, Maciej ZABEL, Paul MOZDZIAK a Bartosz KEMPISTY. Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation. Journal of Clinical Medicine. BASEL: MDPI, 2019, roč. 8, č. 12, s. 1-18. ISSN 2077-0383. Dostupné z: https://dx.doi.org/10.3390/jcm8122026.

Základní údaje

Originální název

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

Autoři

BRAZERT, Maciej (616 Polsko), Wieslawa KRANC (616 Polsko), Blazej CHERMULA (616 Polsko), Katarzyna KOWALSKA (616 Polsko), Maurycy JANKOWSKI (616 Polsko), Piotr CELICHOWSKI (616 Polsko), Michal JEŠETA (203 Česká republika, domácí), Hanna PIOTROWSKA-KEMPISTY (616 Polsko), Leszek PAWELCZYK (616 Polsko), Maciej ZABEL (616 Polsko), Paul MOZDZIAK (616 Polsko) a Bartosz KEMPISTY (616 Polsko, garant)

Vydání

Journal of Clinical Medicine, BASEL, MDPI, 2019, 2077-0383

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.303

Kód RIV

RIV/00216224:14110/19:00112837

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/jcm8122026

UT WoS

000506640400002

Klíčová slova anglicky

ovarian granulosa; human; in vitro; cell division

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Granulosa cells (GCs) are a population of somatic cells whose role after ovulation is progesterone production. GCs were collected from patients undergoing controlled ovarian stimulation during an in vitro fertilization procedure, and they were maintained for 1, 7, 15, and 30 days of in vitro primary culture before collection for further gene expression analysis. A study of genes involved in the biological processes of interest was carried out using expression microarrays. To validate the obtained results, Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) was performed. The direction of changes in the expression of the selected genes was confirmed in most of the examples. Six ontological groups ("cell cycle arrest", "cell cycle process", "mitotic spindle organization", "mitotic spindle assembly checkpoint", "mitotic spindle assembly", and "mitotic spindle checkpoint") were analyzed in this study. The results of the microarrays obtained by us allowed us to identify two groups of genes whose expressions were the most upregulated (FAM64A, ANLN, TOP2A, CTGF, CEP55, BIRC5, PRC1, DLGAP5, GAS6, and NDRG1) and the most downregulated (EREG, PID1, INHA, RHOU, CXCL8, SEPT6, EPGN, RDX, WNT5A, and EZH2) during the culture. The cellular ultrastructure showed the presence of structures characteristic of mitotic cell division: a centrosome surrounded by a pericentric matrix, a microtubule system, and a mitotic spindle connected to chromosomes. The main goal of the study was to identify the genes involved in mitotic division and to identify the cellular ultrastructure of GCs in a long-term in vitro culture. All of the genes in these groups were subjected to downstream analysis, and their function and relation to the ovarian environment are discussed. The obtained results suggest that long-term in vitro cultivation of GCs may lead to their differentiation toward another cell type, including cells with cancer-like characteristics.