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@article{1614756,
author = {Hloušková, Petra and Mandáková, Terezie and Pouch, Milan and Trávníček, Pavel and Lysák, Martin},
article_location = {Oxford},
article_number = {1},
doi = {http://dx.doi.org/10.1093/aob/mcz036},
keywords = {Genome size evolution; repetitive DNA; tandem repeats; retrotransposons; interstitial telomeric repeats (ITRs); chromosome organization; Bunias; Hesperis; Matthiola; Lineage III; Brassicaceae},
language = {eng},
issn = {0305-7364},
journal = {Annals of Botany},
title = {The large genome size variation in the Hesperis clade was shaped by the prevalent proliferation of DNA repeats and rarer genome downsizing},
url = {https://academic.oup.com/aob/article-abstract/124/1/103/5445375?redirectedFrom=fulltext},
volume = {124},
year = {2019}
}
TY - JOUR
ID - 1614756
AU - Hloušková, Petra - Mandáková, Terezie - Pouch, Milan - Trávníček, Pavel - Lysák, Martin
PY - 2019
TI - The large genome size variation in the Hesperis clade was shaped by the prevalent proliferation of DNA repeats and rarer genome downsizing
JF - Annals of Botany
VL - 124
IS - 1
SP - 103-119
EP - 103-119
PB - Oxford University Press
SN - 03057364
KW - Genome size evolution
KW - repetitive DNA
KW - tandem repeats
KW - retrotransposons
KW - interstitial telomeric repeats (ITRs)
KW - chromosome organization
KW - Bunias
KW - Hesperis
KW - Matthiola
KW - Lineage III
KW - Brassicaceae
UR - https://academic.oup.com/aob/article-abstract/124/1/103/5445375?redirectedFrom=fulltext
L2 - https://academic.oup.com/aob/article-abstract/124/1/103/5445375?redirectedFrom=fulltext
N2 - Background and Aims Most crucifer species (Brassicaceae) have small nuclear genomes (mean 1C-value 617 Mb). The species with the largest genomes occur within the monophyletic Hesperis clade (Mandakova et al., Plant Physiology 174: 2062-2071; also known as Clade E or Lineage III). Whereas most chromosome numbers in the clade are 6 or 7, monoploid genome sizes vary 16-fold (256-4264 Mb). To get an insight into genome size evolution in the Hesperis clade (similar to 350 species in similar to 48 genera), we aimed to identify, quantify and localize in situ the repeats from which these genomes are built. We analysed nuclear repeatomes in seven species, covering the phylogenetic and genome size breadth of the clade, by low-pass whole-genome sequencing. Methods Genome size was estimated by flow cytometry. Genomic DNA was sequenced on an Illumina sequencer and DNA repeats were identified and quantified using Repeat Explorer; the most abundant repeats were localized on chromosomes by fluorescence in situ hybridization. To evaluate the feasibility of bacterial artificial chromosome (BAC)-based comparative chromosome painting in Hesperis-clade species, BACs of arabidopsis were used as painting probes. Key Results Most biennial and perennial species of the Hesperis clade possess unusually large nuclear genomes due to the proliferation of long terminal repeat retrotransposons. The prevalent genome expansion was rarely, but repeatedly, counteracted by purging of transposable elements in ephemeral and annual species. Conclusions The most common ancestor of the Hesperis clade has experienced genome upsizing due to transposable element amplification. Further genome size increases, dominating diversification of all Hesperis-clade tribes, contrast with the overall stability of chromosome numbers. In some subclades and species genome downsizing occurred, presumably as an adaptive transition to an annual life cycle. The amplification versus purging of transposable elements and tandem repeats impacted the chromosomal architecture of the Hesperis-clade species.
ER -
HLOUŠKOVÁ, Petra, Terezie MANDÁKOVÁ, Milan POUCH, Pavel TRÁVNÍČEK and Martin LYSÁK. The large genome size variation in the Hesperis clade was shaped by the prevalent proliferation of DNA repeats and rarer genome downsizing. \textit{Annals of Botany}. Oxford: Oxford University Press, 2019, vol.~124, No~1, p.~103-119. ISSN~0305-7364. Available from: https://dx.doi.org/10.1093/aob/mcz036.
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