| CHMÚRČIAKOVÁ, Nikola, Martin KAŠNÝ and Martina OROSOVÁ. Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location. Parasitology International. Clare: Elsevier Ireland Ltd., 2020, vol. 76, JUN 2020, p. 1-8. ISSN 1383-5769. Available from: https://dx.doi.org/10.1016/j.parint.2019.102031. |
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@article{1614956,
author = {Chmúrčiaková, Nikola and Kašný, Martin and Orosová, Martina},
article_location = {Clare},
article_number = {JUN 2020},
doi = {http://dx.doi.org/10.1016/j.parint.2019.102031},
keywords = {Chromosomes; Evolution; Meiosis; Heterochromatin; Fluorescent in situ hybridization},
language = {eng},
issn = {1383-5769},
journal = {Parasitology International},
title = {Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location},
url = {https://www.sciencedirect.com/science/article/pii/S1383576919303824},
volume = {76},
year = {2020}
}
TY - JOUR
ID - 1614956
AU - Chmúrčiaková, Nikola - Kašný, Martin - Orosová, Martina
PY - 2020
TI - Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location
JF - Parasitology International
VL - 76
IS - JUN 2020
SP - 1-8
EP - 1-8
PB - Elsevier Ireland Ltd.
SN - 13835769
KW - Chromosomes
KW - Evolution
KW - Meiosis
KW - Heterochromatin
KW - Fluorescent in situ hybridization
UR - https://www.sciencedirect.com/science/article/pii/S1383576919303824
L2 - https://www.sciencedirect.com/science/article/pii/S1383576919303824
N2 - Ectoparasitic monogeneans of the family Diplozoidae have direct and monoxenous life cycle. The cytogenetics of monogeneans in general and diplozoids in particular, is a relatively underexplored area. This is why each new detailed description of a karyotype provides significant information about the evolution of monogenean chromosomes and contributes to a better understanding of phylogenetic relationships within this group. This study offers new data on the chromosomes of Eudiplozoon nipponicum, an invasive parasite of the common carp. This species' karyotype consists of seven pairs of telocentric chromosomes (2n=14 t). After DAPI staining, we marked heterochromatin blocks on all chromosomes in the pericentromeric region. Silver staining (AgNO3) and staining with fluorescent dye YOYO-1 revealed the presence of one large active nucleolus. Fluorescent in situ hybridization with an 18S rDNA probe revealed one cluster of ribosomal genes at the terminal part of the long arms of chromosome pair No. 7. We compared our results with studies on the phylogenetic relationships of diplozoids which applied a combination of molecular methods and classical morphological characterization and found that the results of our cytogenetic analysis are consistent with the hypothesis that E. nipponicum is more basal member of the family Diplozoidae.
ER -
CHMÚRČIAKOVÁ, Nikola, Martin KAŠNÝ and Martina OROSOVÁ. Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location. \textit{Parasitology International}. Clare: Elsevier Ireland Ltd., 2020, vol.~76, JUN 2020, p.~1-8. ISSN~1383-5769. Available from: https://dx.doi.org/10.1016/j.parint.2019.102031.
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