Identification of microRNA signatures in umbilical cord blood
associated with maternal characteristics

J 2019

Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics

JURÁČEK, Jaroslav, Pavel PILER, Petr JANKŮ, Lenka RADOVÁ, Ondřej SLABÝ et. al.

Basic information

Original name

Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics

Authors

JURÁČEK, Jaroslav (203 Czech Republic, belonging to the institution), Pavel PILER (203 Czech Republic, belonging to the institution), Petr JANKŮ (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

PeerJ, London, PEERJ INC, 2019, 2167-8359

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.379

RIV identification code

RIV/00216224:14740/19:00112874

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.7717/peerj.6981

UT WoS

000469410700002

Keywords in English

Umbilical cord blood; microRNAs; Maternal characteristics

Abstract

V originále

Background. Umbilical cord blood could serve as useful source of blood markers enabling more efficient and reliable prenatal and neonatal diagnostics. MicroRNAs (miRNAs) are ubiquitous in body fluids where they were used for detecting and monitoring various physiological and pathological conditions. In this descriptive study, we aimed to identify changes in miRNA expression profiles associated with basic maternal somatic and epidemiological characteristics. Methods. Study is based on 24 mothers from the Pilot phase of CELSPAC: TNG (Central European Longitudinal Studies of Parents and Children: The Next Generation) study. Cord blood was collected at time of delivery and global miRNA profiling was performed using microRNA Ready-to-use PCR Human Panel I+II TaqMan microarrays. Expression profiles were statistically evaluated in relation to maternal age, BMI, pregnancy weight gain, blood type, Rh factor status, allergies during pregnancy, addictive substance abuse and smoking status. Results. We analyzed expression of 752 human mature miRNAs in 24 samples of umbilical cord blood. For all maternal characteristics tested we described a specific signature of significantly deregulated miRNAs (P < 0.05). Analysis revealed seven miRNA associated with maternal age (three increased and four decreased in women younger than 35 years), 14 miRNAs associated with BMI status (five miRNAs increased and nine miRNAs decreased in women with BMI > 25) and nine miRNAs associated with maternal weight gain during pregnancy (eight miRNAs increased, and one miRNA decreased in women with weight gain < 12 kg). Additionally, 17 miRNAs correlated to blood type (two miRNAs decreased in blood type A, 11 increased in blood type B, two miRNAs increased in blood type AB and two miRNAs increased in blood type 0) and 17 miRNAs to Rh status of mother. We also detected seven miRNAs deregulated in umbilical cord blood of women with allergy (four increased and three decreased in women with allergy), four miRNAs associated to addictive substance abuse status (two up- and two downregulated in women with addictive substance abuse) and eight miRNAs associated with maternal cigarette smoking during pregnancy. Conclusions. We successfully described differences in miRNA profiles in umbilical cord blood associated with basic characteristics connected with mother. Our data suggest that miRNAs in umbilical cord blood are detectable and associated with a wide range of maternal characteristics. These results indicate that miRNAs could potentially serve, and should be studied, as biomarkers for screening and diagnosis of pregnancy-associated complications and pathologies.

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

JURÁČEK, Jaroslav, Pavel PILER, Petr JANKŮ, Lenka RADOVÁ and Ondřej SLABÝ. Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics. PeerJ. London: PEERJ INC, 2019, vol. 7, MAY, p. 6981-6991. ISSN 2167-8359. Available from: https://dx.doi.org/10.7717/peerj.6981.

@article{1617017,
   author = {Juráček, Jaroslav and Piler, Pavel and Janků, Petr and Radová, Lenka and Slabý, Ondřej},
   article_location = {London},
   article_number = {MAY},
   doi = {http://dx.doi.org/10.7717/peerj.6981},
   keywords = {Umbilical cord blood; microRNAs; Maternal characteristics},
   language = {eng},
   issn = {2167-8359},
   journal = {PeerJ},
   title = {Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics},
   url = {https://peerj.com/articles/6981.pdf},
   volume = {7},
   year = {2019}
}

TY  - JOUR
ID  - 1617017
AU  - Juráček, Jaroslav - Piler, Pavel - Janků, Petr - Radová, Lenka - Slabý, Ondřej
PY  - 2019
TI  - Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics
JF  - PeerJ
VL  - 7
IS  - MAY
SP  - 6981
EP  - 6981
PB  - PEERJ INC
SN  - 21678359
KW  - Umbilical cord blood
KW  - microRNAs
KW  - Maternal characteristics
UR  - https://peerj.com/articles/6981.pdf
L2  - https://peerj.com/articles/6981.pdf
N2  - Background. Umbilical cord blood could serve as useful source of blood markers enabling more efficient and reliable prenatal and neonatal diagnostics. MicroRNAs (miRNAs) are ubiquitous in body fluids where they were used for detecting and monitoring various physiological and pathological conditions. In this descriptive study, we aimed to identify changes in miRNA expression profiles associated with basic maternal somatic and epidemiological characteristics. Methods. Study is based on 24 mothers from the Pilot phase of CELSPAC: TNG (Central European Longitudinal Studies of Parents and Children: The Next Generation) study. Cord blood was collected at time of delivery and global miRNA profiling was performed using microRNA Ready-to-use PCR Human Panel I+II TaqMan microarrays. Expression profiles were statistically evaluated in relation to maternal age, BMI, pregnancy weight gain, blood type, Rh factor status, allergies during pregnancy, addictive substance abuse and smoking status. Results. We analyzed expression of 752 human mature miRNAs in 24 samples of umbilical cord blood. For all maternal characteristics tested we described a specific signature of significantly deregulated miRNAs (P < 0.05). Analysis revealed seven miRNA associated with maternal age (three increased and four decreased in women younger than 35 years), 14 miRNAs associated with BMI status (five miRNAs increased and nine miRNAs decreased in women with BMI > 25) and nine miRNAs associated with maternal weight gain during pregnancy (eight miRNAs increased, and one miRNA decreased in women with weight gain < 12 kg). Additionally, 17 miRNAs correlated to blood type (two miRNAs decreased in blood type A, 11 increased in blood type B, two miRNAs increased in blood type AB and two miRNAs increased in blood type 0) and 17 miRNAs to Rh status of mother. We also detected seven miRNAs deregulated in umbilical cord blood of women with allergy (four increased and three decreased in women with allergy), four miRNAs associated to addictive substance abuse status (two up- and two downregulated in women with addictive substance abuse) and eight miRNAs associated with maternal cigarette smoking during pregnancy. Conclusions. We successfully described differences in miRNA profiles in umbilical cord blood associated with basic characteristics connected with mother. Our data suggest that miRNAs in umbilical cord blood are detectable and associated with a wide range of maternal characteristics. These results indicate that miRNAs could potentially serve, and should be studied, as biomarkers for screening and diagnosis of pregnancy-associated complications and pathologies.
ER  -

JURÁČEK, Jaroslav, Pavel PILER, Petr JANKŮ, Lenka RADOVÁ and Ondřej SLABÝ. Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics. \textit{PeerJ}. London: PEERJ INC, 2019, vol.~7, MAY, p.~6981-6991. ISSN~2167-8359. Available from: https://dx.doi.org/10.7717/peerj.6981.

Detailed Information on Publication Record