Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant Viability and Fertility

@article{1618160,
   author = {Zelkowski, M. and Zelkowska, K. and Conrad, U. and Hesse, S. and Lermontova, Inna and Marzec, M. and Meister, A. and Houben, A. and Schubert, V.},
   article_location = {Lausanne (Switzerland)},
   article_number = {JUN},
   doi = {http://dx.doi.org/10.3389/fpls.2019.00774},
   keywords = {Arabidopsis thaliana; meiosis; mitosis; NSE4 delta-kleisin; nucleus; phylogeny; SMC5/6 complex; super-resolution microscopy},
   language = {eng},
   issn = {1664-462X},
   journal = {Frontiers in Plant Science},
   title = {Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant Viability and Fertility},
   url = {https://www.frontiersin.org/articles/10.3389/fpls.2019.00774/full},
   volume = {10},
   year = {2019}
}

TY  - JOUR
ID  - 1618160
AU  - Zelkowski, M. - Zelkowska, K. - Conrad, U. - Hesse, S. - Lermontova, Inna - Marzec, M. - Meister, A. - Houben, A. - Schubert, V.
PY  - 2019
TI  - Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant Viability and Fertility
JF  - Frontiers in Plant Science
VL  - 10
IS  - JUN
SP  - 774
EP  - 774
PB  - Frontiers Media SA
SN  - 1664462X
KW  - Arabidopsis thaliana
KW  - meiosis
KW  - mitosis
KW  - NSE4 delta-kleisin
KW  - nucleus
KW  - phylogeny
KW  - SMC5/6 complex
KW  - super-resolution microscopy
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2019.00774/full
L2  - https://www.frontiersin.org/articles/10.3389/fpls.2019.00774/full
N2  - The SMC 5/6 complex together with cohesin and condensin is a member of the structural maintenance of chromosome (SMC) protein family. In non-plant organisms SMC5/6 is engaged in DNA repair, meiotic synapsis, genome organization and stability. In plants, the function of SMC5/6 is still enigmatic. Therefore, we analyzed the crucial delta-kleisin component NSE4 of the SMC5/6 complex in the model plant Arabidopsis thaliana. Two functional conserved Nse4 paralogs (Nse4A and Nse4B) are present in A. thaliana, which may have evolved via gene subfunctionalization. Due to its high expression level, Nse4A seems to be the more essential gene, whereas Nse4B appears to be involved mainly in seed development. The morphological characterization of A. thaliana T-DNA mutants suggests that the NSE4 proteins are essential for plant growth and fertility. Detailed investigations in wild-type and the mutants based on live cell imaging of transgenic GFP lines, fluorescence in situ hybridization (FISH), immunolabeling and super-resolution microscopy suggest that NSE4A acts in several processes during plant development, such as mitosis, meiosis and chromatin organization of differentiated nuclei, and that NSE4A operates in a cell cycle-dependent manner. Differential response of NSE4A and NSE4B mutants after induced DNA double strand breaks (DSBs) suggests their involvement in DNA repair processes.
ER  - 

ZELKOWSKI, M., K. ZELKOWSKA, U. CONRAD, S. HESSE, Inna LERMONTOVA, M. MARZEC, A. MEISTER, A. HOUBEN a V. SCHUBERT. Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant Viability and Fertility. \textit{Frontiers in Plant Science}. Lausanne (Switzerland): Frontiers Media SA, 2019, roč.~10, JUN, s.~774-793. ISSN~1664-462X. Dostupné z: https://dx.doi.org/10.3389/fpls.2019.00774.