Follicle-stimulating hormone administration affects amino acid
metabolism in mammalian oocytes

J 2019

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA et. al.

Základní údaje

Originální název

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

Autoři

TETKOVA, Anna (203 Česká republika), Andrej SUSOR (203 Česká republika, garant), Michal KUBELKA (203 Česká republika), Lucie NEMCOVA (203 Česká republika), Denisa JANSOVA (203 Česká republika), Michal DVORAN (203 Česká republika), Edgar DEL LLANO (203 Česká republika), Zuzana HOLUBCOVÁ (203 Česká republika, domácí) a Jaroslav KALOUS (203 Česká republika)

Vydání

Biology of reproduction, CARY, OXFORD UNIV PRESS INC, 2019, 0006-3363

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10604 Reproductive biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.322

Kód RIV

RIV/00216224:14110/19:00112932

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1093/biolre/ioz117

UT WoS

000509503400008

Klíčová slova anglicky

oocyte; oocyte maturation; FSH/FSH receptor; translation

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 2. 2020 14:41, Mgr. Tereza Miškechová

Anotace

V originále

Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.

Citovat

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA, Michal DVORAN, Edgar DEL LLANO, Zuzana HOLUBCOVÁ a Jaroslav KALOUS. Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes. Biology of reproduction. CARY: OXFORD UNIV PRESS INC, 2019, roč. 101, č. 4, s. 719-732. ISSN 0006-3363. Dostupné z: https://dx.doi.org/10.1093/biolre/ioz117.

@article{1621517,
   author = {Tetkova, Anna and Susor, Andrej and Kubelka, Michal and Nemcova, Lucie and Jansova, Denisa and Dvoran, Michal and Del Llano, Edgar and Holubcová, Zuzana and Kalous, Jaroslav},
   article_location = {CARY},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/biolre/ioz117},
   keywords = {oocyte; oocyte maturation; FSH/FSH receptor; translation},
   language = {eng},
   issn = {0006-3363},
   journal = {Biology of reproduction},
   title = {Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes},
   url = {http://dx.doi.org/10.1093/biolre/ioz117},
   volume = {101},
   year = {2019}
}

TY  - JOUR
ID  - 1621517
AU  - Tetkova, Anna - Susor, Andrej - Kubelka, Michal - Nemcova, Lucie - Jansova, Denisa - Dvoran, Michal - Del Llano, Edgar - Holubcová, Zuzana - Kalous, Jaroslav
PY  - 2019
TI  - Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes
JF  - Biology of reproduction
VL  - 101
IS  - 4
SP  - 719-732
EP  - 719-732
PB  - OXFORD UNIV PRESS INC
SN  - 00063363
KW  - oocyte
KW  - oocyte maturation
KW  - FSH/FSH receptor
KW  - translation
UR  - http://dx.doi.org/10.1093/biolre/ioz117
L2  - http://dx.doi.org/10.1093/biolre/ioz117
N2  - Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.
ER  -

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA, Michal DVORAN, Edgar DEL LLANO, Zuzana HOLUBCOVÁ a Jaroslav KALOUS. Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes. \textit{Biology of reproduction}. CARY: OXFORD UNIV PRESS INC, 2019, roč.~101, č.~4, s.~719-732. ISSN~0006-3363. Dostupné z: https://dx.doi.org/10.1093/biolre/ioz117.

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