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@article{1623896, author = {Brazert, Maciej and Kranc, Wieslawa and Celichowski, Piotr and Ozegowska, Katarzyna and BudnaandTukan, Joanna and Ješeta, Michal and Pawelczyk, Leszek and Bruska, Malgorzata and Zabel, Maciej and Nowicki, Michal and Kempisty, Bartosz}, article_location = {ATHENS}, article_number = {5}, doi = {http://dx.doi.org/10.3892/mmr.2019.10709}, keywords = {ovarian granulosa; osteoblast; differentiation; microarray; in vitro}, language = {eng}, issn = {1791-2997}, journal = {Molecular Medicine Reports}, title = {Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach}, url = {http://dx.doi.org/10.3892/mmr.2019.10709}, volume = {20}, year = {2019} }
TY - JOUR ID - 1623896 AU - Brazert, Maciej - Kranc, Wieslawa - Celichowski, Piotr - Ozegowska, Katarzyna - Budna-Tukan, Joanna - Ješeta, Michal - Pawelczyk, Leszek - Bruska, Malgorzata - Zabel, Maciej - Nowicki, Michal - Kempisty, Bartosz PY - 2019 TI - Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach JF - Molecular Medicine Reports VL - 20 IS - 5 SP - 4403-4414 EP - 4403-4414 PB - SPANDIDOS PUBL LTD SN - 17912997 KW - ovarian granulosa KW - osteoblast KW - differentiation KW - microarray KW - in vitro UR - http://dx.doi.org/10.3892/mmr.2019.10709 L2 - http://dx.doi.org/10.3892/mmr.2019.10709 N2 - Under physiological conditions, human ovarian granulosa cells (GCs), are responsible for a number of processes associated with folliculogenesis and oogenesis. The primary functions of GCs in the individual phases of follicle growth are: Hormone production in response to follicle stimulating hormone (FSH), induction of ovarian follicle atresia through specific molecular markers and production of nexus cellular connections for communication with the oocyte. In recent years, interest in obtaining stem cells from particular tissues, including the ovary, has increased. Special attention has been paid to the novel properties of GCs during long-term in vitro culture. It has been demonstrated that the usually recycled material in the form of follicular fluid can be a source of cells with stem-like properties. The study group consisted of patients enrolled in the in vitro fertilization procedure. Total RNA was isolated from GCs at 4 time points (after 1, 7, 15 and 30 days of culture) and was used for microarray expression analysis (Affymetrix (R) Human HgU 219 Array). The expression of 22,480 transcripts was examined. The selection of significantly altered genes was based on a P-value <0.05 and expression higher than two-fold. The leucine rich repeat containing 17, collagen type I alpha 1 chain, bone morphogenetic protein 4, twist family bHLH transcription factor 1, insulin like growth factor binding protein 5, GLI family zinc finger 2 and collagen triple helix repeat containing genes exhibited the highest changes in expression. Reverse-transcription-quantitative PCR was performed to validate the results obtained in the analysis of expression microarrays. The direction of expression changes was validated in the majority of cases. The presented results indicated that GCs have the potential of cells that can differentiate towards osteoblasts in long-term in vitro culture conditions. Increased expression of genes associated with the osteogenesis process suggests a potential for uninduced change of GC properties towards the osteoblast phenotype. The present study, therefore, suggests that GCs may become an excellent starting material in obtaining stable osteoblast cultures. GCs differentiated towards osteoblasts may be used in regenerative and reconstructive medicine in the future. ER -
BRAZERT, Maciej, Wieslawa KRANC, Piotr CELICHOWSKI, Katarzyna OZEGOWSKA, Joanna BUDNA-TUKAN, Michal JEŠETA, Leszek PAWELCZYK, Malgorzata BRUSKA, Maciej ZABEL, Michal NOWICKI a Bartosz KEMPISTY. Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach. \textit{Molecular Medicine Reports}. ATHENS: SPANDIDOS PUBL LTD, 2019, roč.~20, č.~5, s.~4403-4414. ISSN~1791-2997. Dostupné z: https://dx.doi.org/10.3892/mmr.2019.10709.
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