2019
Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology
NERADIL, Jakub, Michal KÝR, Kristýna POLÁŠKOVÁ, Leoš KŘEN, Petra MACIGOVÁ et. al.Základní údaje
Originální název
Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology
Autoři
NERADIL, Jakub (203 Česká republika, domácí), Michal KÝR (203 Česká republika, domácí), Kristýna POLÁŠKOVÁ (203 Česká republika, domácí), Leoš KŘEN (203 Česká republika, domácí), Petra MACIGOVÁ (203 Česká republika, domácí), Jan ŠKODA (203 Česká republika, domácí), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Renata VESELSKÁ (203 Česká republika, garant, domácí)
Vydání
Frontiers in Oncology, Lausanne, Frontiers Media SA, 2019, 2234-943X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30204 Oncology
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 4.848
Kód RIV
RIV/00216224:14110/19:00108599
Organizační jednotka
Lékařská fakulta
UT WoS
000487240200001
Klíčová slova anglicky
low-molecular-weight inhibitors; pediatric solid tumors; phospho-protein arrays; phosphorylation profiling; receptor tyrosin kinases; signal transduction
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 11. 5. 2020 13:32, Mgr. Tereza Miškechová
Anotace
V originále
The specific targeting of signal transduction by low-molecular-weight inhibitors or monoclonal antibodies represents a very promising personalized treatment strategy in pediatric oncology. In this study, we present the successful and clinically relevant use of commercially available phospho-protein arrays for analyses of the phosphorylation profiles of a broad spectrum of receptor tyrosine kinases and their downstream signaling proteins in tumor tissue samples. Although these arrays were made for research purposes on human biological samples, they have already been used by several authors to profile various tumor types. Our study performed a systematic analysis of the advantages and pitfalls of the use of this method for personalized clinical medicine. In certain clinical cases and their series, we demonstrated the important aspects of data processing and evaluation, the use of phospho-protein arrays for single sample and serial sample analyses, and the validation of obtained results by immunohistochemistry, as well as the possibilities of this method for the hierarchical clustering of pediatric solid tumors. Our results clearly show that phospho-protein arrays are apparently useful for the clinical consideration of druggable molecular targets within a specific tumor. Thus, their potential validation for diagnostic purposes may substantially improve the personalized approach in the treatment of relapsed or refractory solid tumors.
Návaznosti
MUNI/A/1586/2018, interní kód MU |
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NV16-34083A, projekt VaV |
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