KAHOUNOVA, Zuzana, Eva SLABAKOVA, Lucia BINO, Jan REMSIK, Radek FEDR, Jan BOUCHAL, Radek VRTEL, Lucie JUREČKOVÁ, Volodymyr POROKH, Darja PARALOVA, Aleš HAMPL and Karel SOUCEK. Generation of human iPSCs from fetal prostate fibroblasts HPrF. Stem cell research. Amsterdam: Elsevier, 2019, vol. 35, MAR 2019, p. 1-5. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2019.101405.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Generation of human iPSCs from fetal prostate fibroblasts HPrF
Authors KAHOUNOVA, Zuzana (203 Czech Republic), Eva SLABAKOVA (203 Czech Republic), Lucia BINO (203 Czech Republic), Jan REMSIK (203 Czech Republic), Radek FEDR (203 Czech Republic), Jan BOUCHAL (203 Czech Republic), Radek VRTEL (203 Czech Republic), Lucie JUREČKOVÁ (203 Czech Republic, belonging to the institution), Volodymyr POROKH (804 Ukraine, belonging to the institution), Darja PARALOVA (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Karel SOUCEK (203 Czech Republic, guarantor).
Edition Stem cell research, Amsterdam, Elsevier, 2019, 1873-5061.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.495
RIV identification code RIV/00216224:14110/19:00108162
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.scr.2019.101405
UT WoS 000459831100019
Keywords in English iPSCs; fetal prostate fibroblasts
Tags CF CELLIM, EDITORIAL, rivok
Tags International impact
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 14/4/2020 13:44.
Abstract
Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
Links
GA15-11707S, research and development projectName: Centrosomální abnormality u lidských pluripotentních kmenových buněk.
Investor: Czech Science Foundation
LM2015062, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/C/1418/2017, interní kód MUName: Extracelulární faktory ovlivňující metabolismus centrozomů v lidských pluripotentních kmenových buňkách
Investor: Masaryk University, Rector's Program
PrintDisplayed: 26/5/2024 06:10