The effect of different fatty acid sources on wound healing in
rats assessed by matrix-assisted-laser-desorption-ionization
mass-spectroscopy-imaging

J 2019

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

KOMPRDA, Tomas; Zbysek SLADEK; Veronika SVEHLOVA; Zuzana LACKOVA; Roman GURAN et. al.

Basic information

Original name

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Authors

KOMPRDA, Tomas (203 Czech Republic, guarantor); Zbysek SLADEK (203 Czech Republic); Veronika SVEHLOVA (203 Czech Republic); Zuzana LACKOVA (203 Czech Republic); Roman GURAN (203 Czech Republic); Tomas DO (203 Czech Republic); Jan WIJACKI (203 Czech Republic); Marcela BUCHTOVA (203 Czech Republic); Jana NEUWIRTHOVÁ (203 Czech Republic, belonging to the institution); Břetislav GÁL (203 Czech Republic, belonging to the institution); Vojtech ADAM (203 Czech Republic) and Ondrej ZITKA (203 Czech Republic)

Edition

Acta veterinaria (Brno), Brno, VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2019, 0001-7213

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

40301 Veterinary science

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 0.566

RIV identification code

RIV/00216224:14110/19:00113007

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.2754/avb201988040443

UT WoS

000509758100011

EID Scopus

2-s2.0-85078870875

Keywords in English

Docosahexaenoic acid; eicosapentaenoic acid; Schizochytrium oil; reepithelialization; MALDI MSI; collagen alpha-1(III)

Abstract

V originále

The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Cite

KOMPRDA, Tomas; Zbysek SLADEK; Veronika SVEHLOVA; Zuzana LACKOVA; Roman GURAN; Tomas DO; Jan WIJACKI; Marcela BUCHTOVA; Jana NEUWIRTHOVÁ; Břetislav GÁL; Vojtech ADAM and Ondrej ZITKA. The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging. Acta veterinaria (Brno). Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2019, vol. 88, No 4, p. 443-451. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201988040443.

@article{1625057,
   author = {Komprda, Tomas and Sladek, Zbysek and Svehlova, Veronika and Lackova, Zuzana and Guran, Roman and Do, Tomas and Wijacki, Jan and Buchtova, Marcela and Neuwirthová, Jana and Gál, Břetislav and Adam, Vojtech and Zitka, Ondrej},
   article_location = {Brno},
   article_number = {4},
   doi = {http://dx.doi.org/10.2754/avb201988040443},
   keywords = {Docosahexaenoic acid; eicosapentaenoic acid; Schizochytrium oil; reepithelialization; MALDI MSI; collagen alpha-1(III)},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta veterinaria (Brno)},
   title = {The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging},
   url = {http://dx.doi.org/10.2754/avb201988040443},
   volume = {88},
   year = {2019}
}

TY  - JOUR
ID  - 1625057
AU  - Komprda, Tomas - Sladek, Zbysek - Svehlova, Veronika - Lackova, Zuzana - Guran, Roman - Do, Tomas - Wijacki, Jan - Buchtova, Marcela - Neuwirthová, Jana - Gál, Břetislav - Adam, Vojtech - Zitka, Ondrej
PY  - 2019
TI  - The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging
JF  - Acta veterinaria (Brno)
VL  - 88
IS  - 4
SP  - 443-451
EP  - 443-451
PB  - VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO
SN  - 00017213
KW  - Docosahexaenoic acid
KW  - eicosapentaenoic acid
KW  - Schizochytrium oil
KW  - reepithelialization
KW  - MALDI MSI
KW  - collagen alpha-1(III)
UR  - http://dx.doi.org/10.2754/avb201988040443
L2  - http://dx.doi.org/10.2754/avb201988040443
N2  - The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.
ER  -

KOMPRDA, Tomas; Zbysek SLADEK; Veronika SVEHLOVA; Zuzana LACKOVA; Roman GURAN; Tomas DO; Jan WIJACKI; Marcela BUCHTOVA; Jana NEUWIRTHOVÁ; Břetislav GÁL; Vojtech ADAM and Ondrej ZITKA. The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging. \textit{Acta veterinaria (Brno)}. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2019, vol.~88, No~4, p.~443-451. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb201988040443.

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