A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes
mortality in honey bee pupae (Apis mellifera)

J 2019

A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)

SEITZ, K., K. BUCZOLICH, Alžbeta DIKUNOVÁ, Pavel PLEVKA, K. POWER et. al.

Základní údaje

Originální název

A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)

Autoři

SEITZ, K., K. BUCZOLICH, Alžbeta DIKUNOVÁ (703 Slovensko, domácí), Pavel PLEVKA (203 Česká republika, garant, domácí), K. POWER, T. RIIMENAPF a B. LAMP

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.998

Kód RIV

RIV/00216224:14740/19:00113021

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41598-019-52822-1

UT WoS

000494945700041

Klíčová slova anglicky

PHYLOGENETIC ANALYSIS; GENOME; SUSCEPTIBILITY; IDENTIFICATION; INFECTION; PROTEINS; SPREAD; L.

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 10. 2023 11:07, Mgr. Eva Dubská

Anotace

V originále

Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch's postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.

Citovat

SEITZ, K., K. BUCZOLICH, Alžbeta DIKUNOVÁ, Pavel PLEVKA, K. POWER, T. RIIMENAPF a B. LAMP. A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera). Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, roč. 9, NOV, s. 16274-16284. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-52822-1.

@article{1625870,
   author = {Seitz, K. and Buczolich, K. and Dikunová, Alžbeta and Plevka, Pavel and Power, K. and Riimenapf, T. and Lamp, B.},
   article_location = {LONDON},
   article_number = {NOV},
   doi = {http://dx.doi.org/10.1038/s41598-019-52822-1},
   keywords = {PHYLOGENETIC ANALYSIS; GENOME; SUSCEPTIBILITY; IDENTIFICATION; INFECTION; PROTEINS; SPREAD; L.},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)},
   url = {https://www.nature.com/articles/s41598-019-52822-1.pdf},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1625870
AU  - Seitz, K. - Buczolich, K. - Dikunová, Alžbeta - Plevka, Pavel - Power, K. - Riimenapf, T. - Lamp, B.
PY  - 2019
TI  - A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)
JF  - Scientific reports
VL  - 9
IS  - NOV
SP  - 16274
EP  - 16274
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - PHYLOGENETIC ANALYSIS
KW  - GENOME
KW  - SUSCEPTIBILITY
KW  - IDENTIFICATION
KW  - INFECTION
KW  - PROTEINS
KW  - SPREAD
KW  - L.
UR  - https://www.nature.com/articles/s41598-019-52822-1.pdf
L2  - https://www.nature.com/articles/s41598-019-52822-1.pdf
N2  - Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch's postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.
ER  -

SEITZ, K., K. BUCZOLICH, Alžbeta DIKUNOVÁ, Pavel PLEVKA, K. POWER, T. RIIMENAPF a B. LAMP. A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera). \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, roč.~9, NOV, s.~16274-16284. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-52822-1.

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