Systematic investigation of sequence requirements for DNA
i-motif formation

J 2019

Systematic investigation of sequence requirements for DNA i-motif formation

SKOLAKOVA, P., D. RENCIUK, J. PALACKY, Daniel KRAFČÍK, Z. DVORAKOVA et. al.

Základní údaje

Originální název

Systematic investigation of sequence requirements for DNA i-motif formation

Autoři

SKOLAKOVA, P. (203 Česká republika), D. RENCIUK (203 Česká republika), J. PALACKY (203 Česká republika), Daniel KRAFČÍK (703 Slovensko, garant, domácí), Z. DVORAKOVA (203 Česká republika), I. KEJNOVSKA (203 Česká republika), K. BEDNAROVA (203 Česká republika) a M. VORLICKOVA (203 Česká republika)

Vydání

Nucleic acids research, Oxford, Oxford University Press, 2019, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.502

Kód RIV

RIV/00216224:14740/19:00108184

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/nar/gkz046

UT WoS

000467963700010

Klíčová slova anglicky

GENE-EXPRESSION; RATIONAL DESIGN; G-QUADRUPLEX; LOOP LENGTH; STABILITY; THERMODYNAMICS; KINETICS

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2020 09:23, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The formation of intercalated motifs (iMs) secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is reflected by typical changes in CD and UV absorption spectra. By means of spectroscopic methods, electrophoresis, chemical modifications and other procedures, we characterized iM formation and stability in sequences with different cytosine block lengths interrupted by various numbers and types of nucleotides. Particular attention was paid to the formation of iMs at pH conditions close to neutral. We identified the optimal conditions and minimal requirements for iM formation in DNA sequences, and addressed gaps and inaccurate data interpretations in existing studies to specify principles of iM formation and modes of their folding.

Návaznosti

GA17-12075S, projekt VaV

Název: Polymorfní G-quadruplexy v promotorových oblastech genů

Investor: Grantová agentura ČR, Polymorfní G-quadruplexy v promotorových oblastech genů

Citovat

SKOLAKOVA, P., D. RENCIUK, J. PALACKY, Daniel KRAFČÍK, Z. DVORAKOVA, I. KEJNOVSKA, K. BEDNAROVA a M. VORLICKOVA. Systematic investigation of sequence requirements for DNA i-motif formation. Nucleic acids research. Oxford: Oxford University Press, 2019, roč. 47, č. 5, s. 2177-2189. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkz046.

@article{1635177,
   author = {Skolakova, P. and Renciuk, D. and Palacky, J. and Krafčík, Daniel and Dvorakova, Z. and Kejnovska, I. and Bednarova, K. and Vorlickova, M.},
   article_location = {Oxford},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/nar/gkz046},
   keywords = {GENE-EXPRESSION; RATIONAL DESIGN; G-QUADRUPLEX; LOOP LENGTH; STABILITY; THERMODYNAMICS; KINETICS},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic acids research},
   title = {Systematic investigation of sequence requirements for DNA i-motif formation},
   url = {https://academic.oup.com/nar/article/47/5/2177/5305265},
   volume = {47},
   year = {2019}
}

TY  - JOUR
ID  - 1635177
AU  - Skolakova, P. - Renciuk, D. - Palacky, J. - Krafčík, Daniel - Dvorakova, Z. - Kejnovska, I. - Bednarova, K. - Vorlickova, M.
PY  - 2019
TI  - Systematic investigation of sequence requirements for DNA i-motif formation
JF  - Nucleic acids research
VL  - 47
IS  - 5
SP  - 2177-2189
EP  - 2177-2189
PB  - Oxford University Press
SN  - 03051048
KW  - GENE-EXPRESSION
KW  - RATIONAL DESIGN
KW  - G-QUADRUPLEX
KW  - LOOP LENGTH
KW  - STABILITY
KW  - THERMODYNAMICS
KW  - KINETICS
UR  - https://academic.oup.com/nar/article/47/5/2177/5305265
L2  - https://academic.oup.com/nar/article/47/5/2177/5305265
N2  - The formation of intercalated motifs (iMs) secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is reflected by typical changes in CD and UV absorption spectra. By means of spectroscopic methods, electrophoresis, chemical modifications and other procedures, we characterized iM formation and stability in sequences with different cytosine block lengths interrupted by various numbers and types of nucleotides. Particular attention was paid to the formation of iMs at pH conditions close to neutral. We identified the optimal conditions and minimal requirements for iM formation in DNA sequences, and addressed gaps and inaccurate data interpretations in existing studies to specify principles of iM formation and modes of their folding.
ER  -

SKOLAKOVA, P., D. RENCIUK, J. PALACKY, Daniel KRAFČÍK, Z. DVORAKOVA, I. KEJNOVSKA, K. BEDNAROVA a M. VORLICKOVA. Systematic investigation of sequence requirements for DNA i-motif formation. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2019, roč.~47, č.~5, s.~2177-2189. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkz046.

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