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@article{1636239,
author = {Eberhard, S. and Valuchová, Soňa and Ravat, J. and Fulneček, Jaroslav and Jolivet, P. and Bujaldon, S. and Lemaire, S.D. and Wollman, F.A. and Teixeira, M.T. and Říha, Karel and Xu, Z.},
article_location = {COLD SPRING HARBOR},
article_number = {3},
doi = {http://dx.doi.org/10.26508/lsa.201900315},
keywords = {GENOME-WIDE SCREEN; LENGTH REGULATION; CATALYTIC SUBUNIT; ARABIDOPSIS; YEAST; SEQUENCE; ELONGATION; MECHANISM; EVOLUTION; REPEATS},
language = {eng},
issn = {2575-1077},
journal = {LIFE SCIENCE ALLIANCE},
title = {Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants},
url = {https://www.life-science-alliance.org/content/2/3/e201900315},
volume = {2},
year = {2019}
}
TY - JOUR
ID - 1636239
AU - Eberhard, S. - Valuchová, Soňa - Ravat, J. - Fulneček, Jaroslav - Jolivet, P. - Bujaldon, S. - Lemaire, S.D. - Wollman, F.A. - Teixeira, M.T. - Říha, Karel - Xu, Z.
PY - 2019
TI - Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants
JF - LIFE SCIENCE ALLIANCE
VL - 2
IS - 3
SP - 1-15
EP - 1-15
PB - LIFE SCIENCE ALLIANCE LLC
SN - 25751077
KW - GENOME-WIDE SCREEN
KW - LENGTH REGULATION
KW - CATALYTIC SUBUNIT
KW - ARABIDOPSIS
KW - YEAST
KW - SEQUENCE
KW - ELONGATION
KW - MECHANISM
KW - EVOLUTION
KW - REPEATS
UR - https://www.life-science-alliance.org/content/2/3/e201900315
L2 - https://www.life-science-alliance.org/content/2/3/e201900315
N2 - Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening. Although Chlamydomonas reinhardtii, a photosynthetic unicellular green alga, is widely used as a model organism in photosynthesis and flagella research, and for biotechnological applications, the biology of its telomeres has not been investigated in depth. Here, we show that the C. reinhardtii (TTTTAGGG)(n) telomeric repeats are mostly nondegenerate and that the telomeres form a protective structure, with a subset ending with a 3' overhang and another subset presenting a blunt end. Although telomere size and length distributions are stable under various standard growth conditions, they vary substantially between 12 genetically close reference strains. Finally, we identify CrTERT, the gene encoding the catalytic subunit of telomerase and show that telomeres shorten progressively in mutants of this gene. Telomerase mutants eventually enter replicative senescence, demonstrating that telomerase is required for long-term maintenance of telomeres in C. reinhardtii.
ER -
EBERHARD, S., Soňa VALUCHOVÁ, J. RAVAT, Jaroslav FULNEČEK, P. JOLIVET, S. BUJALDON, S.D. LEMAIRE, F.A. WOLLMAN, M.T. TEIXEIRA, Karel ŘÍHA a Z. XU. Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. \textit{LIFE SCIENCE ALLIANCE}. COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC, 2019, roč.~2, č.~3, s.~1-15. ISSN~2575-1077. Dostupné z: https://dx.doi.org/10.26508/lsa.201900315.
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