Molecular characterization of Chlamydomonas reinhardtii
telomeres and telomerase mutants

J 2019

Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants

EBERHARD, S., Soňa VALUCHOVÁ, J. RAVAT, Jaroslav FULNEČEK, P. JOLIVET et. al.

Základní údaje

Originální název

Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants

Autoři

EBERHARD, S., Soňa VALUCHOVÁ (703 Slovensko, domácí), J. RAVAT, Jaroslav FULNEČEK (203 Česká republika, domácí), P. JOLIVET, S. BUJALDON, S.D. LEMAIRE, F.A. WOLLMAN, M.T. TEIXEIRA, Karel ŘÍHA (203 Česká republika, garant, domácí) a Z. XU

Vydání

LIFE SCIENCE ALLIANCE, COLD SPRING HARBOR, LIFE SCIENCE ALLIANCE LLC, 2019, 2575-1077

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.622

Kód RIV

RIV/00216224:14740/19:00113265

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.26508/lsa.201900315

UT WoS

000473222200015

Klíčová slova anglicky

GENOME-WIDE SCREEN; LENGTH REGULATION; CATALYTIC SUBUNIT; ARABIDOPSIS; YEAST; SEQUENCE; ELONGATION; MECHANISM; EVOLUTION; REPEATS

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2020 11:12, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening. Although Chlamydomonas reinhardtii, a photosynthetic unicellular green alga, is widely used as a model organism in photosynthesis and flagella research, and for biotechnological applications, the biology of its telomeres has not been investigated in depth. Here, we show that the C. reinhardtii (TTTTAGGG)(n) telomeric repeats are mostly nondegenerate and that the telomeres form a protective structure, with a subset ending with a 3' overhang and another subset presenting a blunt end. Although telomere size and length distributions are stable under various standard growth conditions, they vary substantially between 12 genetically close reference strains. Finally, we identify CrTERT, the gene encoding the catalytic subunit of telomerase and show that telomeres shorten progressively in mutants of this gene. Telomerase mutants eventually enter replicative senescence, demonstrating that telomerase is required for long-term maintenance of telomeres in C. reinhardtii.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

Citovat

EBERHARD, S., Soňa VALUCHOVÁ, J. RAVAT, Jaroslav FULNEČEK, P. JOLIVET, S. BUJALDON, S.D. LEMAIRE, F.A. WOLLMAN, M.T. TEIXEIRA, Karel ŘÍHA a Z. XU. Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. LIFE SCIENCE ALLIANCE. COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC, 2019, roč. 2, č. 3, s. 1-15. ISSN 2575-1077. Dostupné z: https://dx.doi.org/10.26508/lsa.201900315.

@article{1636239,
   author = {Eberhard, S. and Valuchová, Soňa and Ravat, J. and Fulneček, Jaroslav and Jolivet, P. and Bujaldon, S. and Lemaire, S.D. and Wollman, F.A. and Teixeira, M.T. and Říha, Karel and Xu, Z.},
   article_location = {COLD SPRING HARBOR},
   article_number = {3},
   doi = {http://dx.doi.org/10.26508/lsa.201900315},
   keywords = {GENOME-WIDE SCREEN; LENGTH REGULATION; CATALYTIC SUBUNIT; ARABIDOPSIS; YEAST; SEQUENCE; ELONGATION; MECHANISM; EVOLUTION; REPEATS},
   language = {eng},
   issn = {2575-1077},
   journal = {LIFE SCIENCE ALLIANCE},
   title = {Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants},
   url = {https://www.life-science-alliance.org/content/2/3/e201900315},
   volume = {2},
   year = {2019}
}

TY  - JOUR
ID  - 1636239
AU  - Eberhard, S. - Valuchová, Soňa - Ravat, J. - Fulneček, Jaroslav - Jolivet, P. - Bujaldon, S. - Lemaire, S.D. - Wollman, F.A. - Teixeira, M.T. - Říha, Karel - Xu, Z.
PY  - 2019
TI  - Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants
JF  - LIFE SCIENCE ALLIANCE
VL  - 2
IS  - 3
SP  - 1-15
EP  - 1-15
PB  - LIFE SCIENCE ALLIANCE LLC
SN  - 25751077
KW  - GENOME-WIDE SCREEN
KW  - LENGTH REGULATION
KW  - CATALYTIC SUBUNIT
KW  - ARABIDOPSIS
KW  - YEAST
KW  - SEQUENCE
KW  - ELONGATION
KW  - MECHANISM
KW  - EVOLUTION
KW  - REPEATS
UR  - https://www.life-science-alliance.org/content/2/3/e201900315
L2  - https://www.life-science-alliance.org/content/2/3/e201900315
N2  - Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening. Although Chlamydomonas reinhardtii, a photosynthetic unicellular green alga, is widely used as a model organism in photosynthesis and flagella research, and for biotechnological applications, the biology of its telomeres has not been investigated in depth. Here, we show that the C. reinhardtii (TTTTAGGG)(n) telomeric repeats are mostly nondegenerate and that the telomeres form a protective structure, with a subset ending with a 3' overhang and another subset presenting a blunt end. Although telomere size and length distributions are stable under various standard growth conditions, they vary substantially between 12 genetically close reference strains. Finally, we identify CrTERT, the gene encoding the catalytic subunit of telomerase and show that telomeres shorten progressively in mutants of this gene. Telomerase mutants eventually enter replicative senescence, demonstrating that telomerase is required for long-term maintenance of telomeres in C. reinhardtii.
ER  -

EBERHARD, S., Soňa VALUCHOVÁ, J. RAVAT, Jaroslav FULNEČEK, P. JOLIVET, S. BUJALDON, S.D. LEMAIRE, F.A. WOLLMAN, M.T. TEIXEIRA, Karel ŘÍHA a Z. XU. Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. \textit{LIFE SCIENCE ALLIANCE}. COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC, 2019, roč.~2, č.~3, s.~1-15. ISSN~2575-1077. Dostupné z: https://dx.doi.org/10.26508/lsa.201900315.

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