Anti-apoptotic and pro-apoptotic factors in human granulosa
cells cultured in 3Dculture system

a 2019

Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3Dculture system

CHMELÍKOVÁ, Eva, M. SEDMÍKOVÁ, Michal JEŠETA, P. POSTLEROVÁ, R. KŘEJČÍŘOVÁ et. al.

Základní údaje

Originální název

Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3Dculture system

Autoři

CHMELÍKOVÁ, Eva (203 Česká republika, garant), M. SEDMÍKOVÁ (203 Česká republika), Michal JEŠETA (203 Česká republika, domácí), P. POSTLEROVÁ (203 Česká republika), R. KŘEJČÍŘOVÁ (203 Česká republika) a T. KREJČOVÁ (203 Česká republika)

Vydání

3rd CellFit Annual Meeting, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/19:00113277

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

human granulosa cells

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 15. 4. 2020 14:38, Mgr. Tereza Miškechová

Anotace

V originále

Granulosa cells have several functions during follicular development; for example, theynurse the oocyte, regulate steroid hormones production and activity, and preventoocyte against apoptosis. For these abilities, granulosa cells are crucial for manyreproductive functions and are suitable for in vitro culture and testing. However, whengranulosa cells are cultured in vitro as monolayers, they gradually lose most of theirimportant functions.The aim of our study was to test the three dimensional (3D) culture system for humangranulosa cells. For this purpose nanofibrous cellulose hydrogel GrowDex was used. Theexpression ofpro-and anti-apoptotic factors in human granulosa cells was investigatedwith the target to verify the usability of the 3D system with GrowDex.Human granulosa cells denuded from oocytes were cultured for 16 days in 4-well NUNCculture dishes with GrowDex. Conventional 2D (monolayer) culture system was used asa control. After prolong 16 days culture the expression of pro-and anti-apoptoticmarkers; Bax and BCl 2 respectively, was examined by Western blotting. The cellscultured in 3D culture system expressed anti-apoptotic factor BCl 2 in higher amountsthan cells cultured in the conventional 2D culture system. Besides, the expression ofpro-apoptotic factor Bax was lower in the 3D system than the expression in theconventional culture system.

Citovat

CHMELÍKOVÁ, Eva, M. SEDMÍKOVÁ, Michal JEŠETA, P. POSTLEROVÁ, R. KŘEJČÍŘOVÁ a T. KREJČOVÁ. Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3Dculture system. In 3rd CellFit Annual Meeting. 2019.

@proceedings{1636618,
   author = {Chmelíková, Eva and Sedmíková, M. and Ješeta, Michal and Postlerová, P. and Křejčířová, R. and Krejčová, T.},
   booktitle = {3rd CellFit Annual Meeting},
   keywords = {human granulosa cells},
   language = {eng},
   title = {Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3Dculture system},
   url = {http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf},
   year = {2019}
}

TY  - CONF
ID  - 1636618
AU  - Chmelíková, Eva - Sedmíková, M. - Ješeta, Michal - Postlerová, P. - Křejčířová, R. - Krejčová, T.
PY  - 2019
TI  - Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3Dculture system
KW  - human granulosa cells
UR  - http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf
L2  - http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf
N2  - Granulosa cells have several functions during follicular development; for example, theynurse the oocyte, regulate steroid hormones production and activity, and preventoocyte against apoptosis. For these abilities, granulosa cells are crucial for manyreproductive functions and are suitable for in vitro culture and testing. However, whengranulosa cells are cultured in vitro as monolayers, they gradually lose most of theirimportant functions.The aim of our study was to test the three dimensional (3D) culture system for humangranulosa cells. For this purpose nanofibrous cellulose hydrogel GrowDex was used. Theexpression ofpro-and anti-apoptotic factors in human granulosa cells was investigatedwith the target to verify the usability of the 3D system with GrowDex.Human granulosa cells denuded from oocytes were cultured for 16 days in 4-well NUNCculture dishes with GrowDex. Conventional 2D (monolayer) culture system was used asa control. After prolong 16 days culture the expression of pro-and anti-apoptoticmarkers; Bax and BCl 2 respectively, was examined by Western blotting. The cellscultured in 3D culture system expressed anti-apoptotic factor BCl 2 in higher amountsthan cells cultured in the conventional 2D culture system. Besides, the expression ofpro-apoptotic factor Bax was lower in the 3D system than the expression in theconventional culture system.
ER  -

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