CdS quantum dots-based immunoassay combined with particle
imprinted polymer technology and laser ablation ICP-MS as a
versatile tool for protein detection

J 2019

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA et. al.

Základní údaje

Originální název

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

Autoři

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ (203 Česká republika, domácí), M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ (203 Česká republika, domácí), Viktor KANICKÝ (203 Česká republika, domácí), V. ADAM, M. VACULOVICOVA a Tomáš VACULOVIČ (203 Česká republika, domácí)

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.998

Kód RIV

RIV/00216224:14310/19:00113292

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-019-48290-2

UT WoS

000480678100039

Klíčová slova anglicky

MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2020 19:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA a Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, roč. 9, AUG, s. 1-9. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-48290-2.

@article{1636928,
   author = {Vaneckova, T. and Bezdekova, J. and Tvrdoňová, Michaela and Vlcnovska, M. and Novotna, V. and Neuman, J. and Štossová, Aneta and Kanický, Viktor and Adam, V. and Vaculovicova, M. and Vaculovič, Tomáš},
   article_location = {LONDON},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.1038/s41598-019-48290-2},
   keywords = {MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection},
   url = {https://www.nature.com/articles/s41598-019-48290-2#Ack1},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1636928
AU  - Vaneckova, T. - Bezdekova, J. - Tvrdoňová, Michaela - Vlcnovska, M. - Novotna, V. - Neuman, J. - Štossová, Aneta - Kanický, Viktor - Adam, V. - Vaculovicova, M. - Vaculovič, Tomáš
PY  - 2019
TI  - CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
JF  - Scientific reports
VL  - 9
IS  - AUG
SP  - 1-9
EP  - 1-9
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - MAGNETIC NANOPARTICLES
KW  - SENSITIVE DETECTION
KW  - SURFACE-CHEMISTRY
KW  - RECOGNITION
KW  - SENSOR
KW  - EXTRACTION
KW  - SEPARATION
KW  - DNA
UR  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
L2  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
N2  - For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.
ER  -

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA a Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, roč.~9, AUG, s.~1-9. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-48290-2.

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