CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, vol. 9, AUG, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-48290-2.

Basic information

• Original name: CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
• Authors: VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ (203 Czech Republic, belonging to the institution), M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution).
• Edition: Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.998
• RIV identification code: RIV/00216224:14310/19:00113292
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1038/s41598-019-48290-2
• UT WoS: 000480678100039
• Keywords in English: MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR