CdS quantum dots-based immunoassay combined with particle
imprinted polymer technology and laser ablation ICP-MS as a
versatile tool for protein detection

J 2019

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA et. al.

Basic information

Original name

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

Authors

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ (203 Czech Republic, belonging to the institution), M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution)

Edition

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.998

RIV identification code

RIV/00216224:14310/19:00113292

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-019-48290-2

UT WoS

000480678100039

Keywords in English

MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA

Abstract

V originále

For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, vol. 9, AUG, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-48290-2.

@article{1636928,
   author = {Vaneckova, T. and Bezdekova, J. and Tvrdoňová, Michaela and Vlcnovska, M. and Novotna, V. and Neuman, J. and Štossová, Aneta and Kanický, Viktor and Adam, V. and Vaculovicova, M. and Vaculovič, Tomáš},
   article_location = {LONDON},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.1038/s41598-019-48290-2},
   keywords = {MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection},
   url = {https://www.nature.com/articles/s41598-019-48290-2#Ack1},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1636928
AU  - Vaneckova, T. - Bezdekova, J. - Tvrdoňová, Michaela - Vlcnovska, M. - Novotna, V. - Neuman, J. - Štossová, Aneta - Kanický, Viktor - Adam, V. - Vaculovicova, M. - Vaculovič, Tomáš
PY  - 2019
TI  - CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
JF  - Scientific reports
VL  - 9
IS  - AUG
SP  - 1-9
EP  - 1-9
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - MAGNETIC NANOPARTICLES
KW  - SENSITIVE DETECTION
KW  - SURFACE-CHEMISTRY
KW  - RECOGNITION
KW  - SENSOR
KW  - EXTRACTION
KW  - SEPARATION
KW  - DNA
UR  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
L2  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
N2  - For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.
ER  -

VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, vol.~9, AUG, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-48290-2.

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