Molecularly imprinted polymers coupled to mass spectrometric
detection for metallothionein sensing

J 2019

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

VANECKOVA, T., L. VANICKOVA, Michaela TVRDOŇOVÁ, A. POMORSKI, A. KREZEL et. al.

Základní údaje

Originální název

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

Autoři

VANECKOVA, T., L. VANICKOVA, Michaela TVRDOŇOVÁ (203 Česká republika, domácí), A. POMORSKI, A. KREZEL, Tomáš VACULOVIČ (203 Česká republika, domácí), Viktor KANICKÝ (203 Česká republika, domácí), M. VACULOVICOVA a V. ADAM

Vydání

Talanta, AMSTERDAM, Elsevier, 2019, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.339

Kód RIV

RIV/00216224:14310/19:00108189

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.talanta.2019.01.089

UT WoS

000462803400030

Klíčová slova anglicky

Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2020 20:25, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).

Návaznosti

GA17-12774S, projekt VaV

Název: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Grantová agentura ČR, Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

VANECKOVA, T., L. VANICKOVA, Michaela TVRDOŇOVÁ, A. POMORSKI, A. KREZEL, Tomáš VACULOVIČ, Viktor KANICKÝ, M. VACULOVICOVA a V. ADAM. Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing. Talanta. AMSTERDAM: Elsevier, 2019, roč. 198, JUN, s. 224-229. ISSN 0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2019.01.089.

@article{1636956,
   author = {Vaneckova, T. and Vanickova, L. and Tvrdoňová, Michaela and Pomorski, A. and Krezel, A. and Vaculovič, Tomáš and Kanický, Viktor and Vaculovicova, M. and Adam, V.},
   article_location = {AMSTERDAM},
   article_number = {JUN},
   doi = {http://dx.doi.org/10.1016/j.talanta.2019.01.089},
   keywords = {Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub},
   volume = {198},
   year = {2019}
}

TY  - JOUR
ID  - 1636956
AU  - Vaneckova, T. - Vanickova, L. - Tvrdoňová, Michaela - Pomorski, A. - Krezel, A. - Vaculovič, Tomáš - Kanický, Viktor - Vaculovicova, M. - Adam, V.
PY  - 2019
TI  - Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing
JF  - Talanta
VL  - 198
IS  - JUN
SP  - 224-229
EP  - 224-229
PB  - Elsevier
SN  - 00399140
KW  - Metallothionein
KW  - Molecularly imprinted polymers
KW  - Polydopamine
KW  - MALDI-TOP-MS
KW  - LA-ICP-MS
UR  - https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub
N2  - We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).
ER  -

VANECKOVA, T., L. VANICKOVA, Michaela TVRDOŇOVÁ, A. POMORSKI, A. KREZEL, Tomáš VACULOVIČ, Viktor KANICKÝ, M. VACULOVICOVA a V. ADAM. Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing. \textit{Talanta}. AMSTERDAM: Elsevier, 2019, roč.~198, JUN, s.~224-229. ISSN~0039-9140. Dostupné z: https://dx.doi.org/10.1016/j.talanta.2019.01.089.

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