Multi-Electrode Array with a Planar Surface for Cell Patterning
by Microprinting

J 2019

Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting

SLAVIK, Jan, Josef SKOPALIK, Ivo PROVAZNÍK a Jaromir HUBALEK

Základní údaje

Originální název

Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting

Autoři

SLAVIK, Jan (203 Česká republika, garant), Josef SKOPALIK (203 Česká republika), Ivo PROVAZNÍK (203 Česká republika, domácí) a Jaromir HUBALEK (203 Česká republika)

Vydání

Sensors, Basel, MDPI, 2019, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.275

Kód RIV

RIV/00216224:14110/19:00113322

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/s19245379

UT WoS

000517961400043

Klíčová slova anglicky

multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 11:18, Mgr. Tereza Miškechová

Anotace

V originále

Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.

Návaznosti

LM2015041, projekt VaV

Název: CEITEC Nano

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC Nano

Citovat

SLAVIK, Jan, Josef SKOPALIK, Ivo PROVAZNÍK a Jaromir HUBALEK. Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting. Sensors. Basel: MDPI, 2019, roč. 19, č. 24, s. 1-11. ISSN 1424-8220. Dostupné z: https://dx.doi.org/10.3390/s19245379.

@article{1637257,
   author = {Slavik, Jan and Skopalik, Josef and Provazník, Ivo and Hubalek, Jaromir},
   article_location = {Basel},
   article_number = {24},
   doi = {http://dx.doi.org/10.3390/s19245379},
   keywords = {multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting},
   url = {http://dx.doi.org/10.3390/s19245379},
   volume = {19},
   year = {2019}
}

TY  - JOUR
ID  - 1637257
AU  - Slavik, Jan - Skopalik, Josef - Provazník, Ivo - Hubalek, Jaromir
PY  - 2019
TI  - Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting
JF  - Sensors
VL  - 19
IS  - 24
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 14248220
KW  - multielectrode array
KW  - microelectrode array
KW  - sacrificial layer
KW  - peel-off
KW  - HL-1 cells
KW  - microprinting
KW  - antifouling agent
UR  - http://dx.doi.org/10.3390/s19245379
L2  - http://dx.doi.org/10.3390/s19245379
N2  - Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.
ER  -

SLAVIK, Jan, Josef SKOPALIK, Ivo PROVAZNÍK a Jaromir HUBALEK. Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting. \textit{Sensors}. Basel: MDPI, 2019, roč.~19, č.~24, s.~1-11. ISSN~1424-8220. Dostupné z: https://dx.doi.org/10.3390/s19245379.

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