Receptor-Independent Transfer of Low Density Lipoprotein Cargo
to Biomembranes

J 2019

Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes

AXMANN, M., E. SEZGIN, A. KARNER, Jiří NOVÁČEK, M.D. BRODESSER et. al.

Základní údaje

Originální název

Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes

Autoři

AXMANN, M., E. SEZGIN, A. KARNER, Jiří NOVÁČEK (203 Česká republika, garant, domácí), M.D. BRODESSER, C. ROHRL, J. PREINER, H. STANGL a B. PLOCHBERGER

Vydání

NANO LETTERS, WASHINGTON, AMER CHEMICAL SOC, 2019, 1530-6984

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.238

Kód RIV

RIV/00216224:14740/19:00113327

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/acs.nanolett.9b00319

UT WoS

000464769100050

Klíčová slova anglicky

Low density lipoprotein; (high-speed) atomic force microscopy; fluorescence (cross) correlation spectroscopy; single-molecule-sensitive imaging; cryo-electron microscopy; cholesterol transfer

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:03, Ing. Martina Blahová

Anotace

V originále

The fundamental task of lipoprotein particles is extracellular transport of cholesterol, lipids, and fatty acids. Besides, cholesterol-rich apoB-containing lipoprotein particles (i.e., low density lipoprotein LDL) are key players in progression of atherosclerotic cardiovascular disease and are associated with familial hypercholesterolemia (FH). So far, lipoprotein particle binding to the cell membrane and subsequent cargo transfer is directly linked to the lipoprotein receptors on the target cell surface. However, our observations showed that lipoprotein particle cargo transport takes place even in the absence of the receptor. This finding suggests that an alternative mechanism for lipoprotein-particle/membrane interaction, besides the receptor-mediated one, exists. Here, we combined several complementary biophysical techniques to obtain a comprehensive view on the nonreceptor mediated LDL-particle/membrane. We applied a combination of atomic force and single-molecule-sensitive fluorescence microscopy (AFM and SMFM) to investigate the LDL particle interaction with membranes of increasing complexity. We observed direct transfer of fluorescently labeled amphiphilic lipid molecules from LDL particles into the pure lipid bilayer. We further confirmed cargo transfer by fluorescence cross-correlation spectroscopy (FCCS) and spectral imaging of environment-sensitive probes. Moreover, the integration of the LDL particle into the membranes was directly visualized by high-speed atomic force microscopy (HS-AFM) and cryo-electron microscopy (cryo-EM). Overall, our data show that lipoprotein particles are able to incorporate into lipid membranes upon contact to transfer their cargo in the absence of specific receptors.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

AXMANN, M., E. SEZGIN, A. KARNER, Jiří NOVÁČEK, M.D. BRODESSER, C. ROHRL, J. PREINER, H. STANGL a B. PLOCHBERGER. Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes. NANO LETTERS. WASHINGTON: AMER CHEMICAL SOC, 2019, roč. 19, č. 4, s. 2562-2567. ISSN 1530-6984. Dostupné z: https://dx.doi.org/10.1021/acs.nanolett.9b00319.

@article{1637377,
   author = {Axmann, M. and Sezgin, E. and Karner, A. and Nováček, Jiří and Brodesser, M.D. and Rohrl, C. and Preiner, J. and Stangl, H. and Plochberger, B.},
   article_location = {WASHINGTON},
   article_number = {4},
   doi = {http://dx.doi.org/10.1021/acs.nanolett.9b00319},
   keywords = {Low density lipoprotein; (high-speed) atomic force microscopy; fluorescence (cross) correlation spectroscopy; single-molecule-sensitive imaging; cryo-electron microscopy; cholesterol transfer},
   language = {eng},
   issn = {1530-6984},
   journal = {NANO LETTERS},
   title = {Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes},
   url = {https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319},
   volume = {19},
   year = {2019}
}

TY  - JOUR
ID  - 1637377
AU  - Axmann, M. - Sezgin, E. - Karner, A. - Nováček, Jiří - Brodesser, M.D. - Rohrl, C. - Preiner, J. - Stangl, H. - Plochberger, B.
PY  - 2019
TI  - Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes
JF  - NANO LETTERS
VL  - 19
IS  - 4
SP  - 2562-2567
EP  - 2562-2567
PB  - AMER CHEMICAL SOC
SN  - 15306984
KW  - Low density lipoprotein
KW  - (high-speed) atomic force microscopy
KW  - fluorescence (cross) correlation spectroscopy
KW  - single-molecule-sensitive imaging
KW  - cryo-electron microscopy
KW  - cholesterol transfer
UR  - https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319
L2  - https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319
N2  - The fundamental task of lipoprotein particles is extracellular transport of cholesterol, lipids, and fatty acids. Besides, cholesterol-rich apoB-containing lipoprotein particles (i.e., low density lipoprotein LDL) are key players in progression of atherosclerotic cardiovascular disease and are associated with familial hypercholesterolemia (FH). So far, lipoprotein particle binding to the cell membrane and subsequent cargo transfer is directly linked to the lipoprotein receptors on the target cell surface. However, our observations showed that lipoprotein particle cargo transport takes place even in the absence of the receptor. This finding suggests that an alternative mechanism for lipoprotein-particle/membrane interaction, besides the receptor-mediated one, exists. Here, we combined several complementary biophysical techniques to obtain a comprehensive view on the nonreceptor mediated LDL-particle/membrane. We applied a combination of atomic force and single-molecule-sensitive fluorescence microscopy (AFM and SMFM) to investigate the LDL particle interaction with membranes of increasing complexity. We observed direct transfer of fluorescently labeled amphiphilic lipid molecules from LDL particles into the pure lipid bilayer. We further confirmed cargo transfer by fluorescence cross-correlation spectroscopy (FCCS) and spectral imaging of environment-sensitive probes. Moreover, the integration of the LDL particle into the membranes was directly visualized by high-speed atomic force microscopy (HS-AFM) and cryo-electron microscopy (cryo-EM). Overall, our data show that lipoprotein particles are able to incorporate into lipid membranes upon contact to transfer their cargo in the absence of specific receptors.
ER  -

AXMANN, M., E. SEZGIN, A. KARNER, Jiří NOVÁČEK, M.D. BRODESSER, C. ROHRL, J. PREINER, H. STANGL a B. PLOCHBERGER. Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes. \textit{NANO LETTERS}. WASHINGTON: AMER CHEMICAL SOC, 2019, roč.~19, č.~4, s.~2562-2567. ISSN~1530-6984. Dostupné z: https://dx.doi.org/10.1021/acs.nanolett.9b00319.

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