The effects of nano-sized PbO on biomarkers of membrane
disruption and DNA damage in a sub-chronic inhalation study on
mice

J 2020

The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL et. al.

Základní údaje

Originální název

The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice

Autoři

BLÁHOVÁ, Lucie (203 Česká republika, domácí), Zuzana NOVÁKOVÁ (203 Česká republika, domácí), Zbyněk VEČEŘA (203 Česká republika), Lucie VRLÍKOVÁ (203 Česká republika), Bohumil DOČEKAL (203 Česká republika), Jana DUMKOVÁ (203 Česká republika, domácí), Kamil KRUMAL (203 Česká republika), Pavel MIKUSKA (203 Česká republika), Marcela BUCHTOVÁ (203 Česká republika, domácí), Aleš HAMPL (203 Česká republika, domácí), Klára HILSCHEROVÁ (203 Česká republika, domácí) a Luděk BLÁHA (203 Česká republika, garant, domácí)

Vydání

NANOTOXICOLOGY, ABINGDON, TAYLOR & FRANCIS LTD, 2020, 1743-5390

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.913

Kód RIV

RIV/00216224:14310/20:00115464

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1080/17435390.2019.1685696

UT WoS

000496628900001

Klíčová slova anglicky

Oxidative damage; lead; nanoparticle; 8-OHdG; TBARS; 8-isoPGF2(alpha); 8-isoPGE isoprostanes; LC-MS; MS; lipid peroxidation; inhalation

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 2. 2023 17:26, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 mu g/m(3); 1.93 x 10(6) particles/cm(3)) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E-2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Jana DUMKOVÁ, Kamil KRUMAL, Pavel MIKUSKA, Marcela BUCHTOVÁ, Aleš HAMPL, Klára HILSCHEROVÁ a Luděk BLÁHA. The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice. NANOTOXICOLOGY. ABINGDON: TAYLOR & FRANCIS LTD, 2020, roč. 14, č. 2, s. 214-231. ISSN 1743-5390. Dostupné z: https://dx.doi.org/10.1080/17435390.2019.1685696.

@article{1640436,
   author = {Bláhová, Lucie and Nováková, Zuzana and Večeřa, Zbyněk and Vrlíková, Lucie and Dočekal, Bohumil and Dumková, Jana and Krumal, Kamil and Mikuska, Pavel and Buchtová, Marcela and Hampl, Aleš and Hilscherová, Klára and Bláha, Luděk},
   article_location = {ABINGDON},
   article_number = {2},
   doi = {http://dx.doi.org/10.1080/17435390.2019.1685696},
   keywords = {Oxidative damage; lead; nanoparticle; 8-OHdG; TBARS; 8-isoPGF2(alpha); 8-isoPGE isoprostanes; LC-MS; MS; lipid peroxidation; inhalation},
   language = {eng},
   issn = {1743-5390},
   journal = {NANOTOXICOLOGY},
   title = {The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice},
   url = {https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20},
   volume = {14},
   year = {2020}
}

TY  - JOUR
ID  - 1640436
AU  - Bláhová, Lucie - Nováková, Zuzana - Večeřa, Zbyněk - Vrlíková, Lucie - Dočekal, Bohumil - Dumková, Jana - Krumal, Kamil - Mikuska, Pavel - Buchtová, Marcela - Hampl, Aleš - Hilscherová, Klára - Bláha, Luděk
PY  - 2020
TI  - The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice
JF  - NANOTOXICOLOGY
VL  - 14
IS  - 2
SP  - 214-231
EP  - 214-231
PB  - TAYLOR & FRANCIS LTD
SN  - 17435390
KW  - Oxidative damage
KW  - lead
KW  - nanoparticle
KW  - 8-OHdG
KW  - TBARS
KW  - 8-isoPGF2(alpha)
KW  - 8-isoPGE isoprostanes
KW  - LC-MS
KW  - MS
KW  - lipid peroxidation
KW  - inhalation
UR  - https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20
L2  - https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20
N2  - Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 mu g/m(3); 1.93 x 10(6) particles/cm(3)) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E-2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.
ER  -

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Jana DUMKOVÁ, Kamil KRUMAL, Pavel MIKUSKA, Marcela BUCHTOVÁ, Aleš HAMPL, Klára HILSCHEROVÁ a Luděk BLÁHA. The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice. \textit{NANOTOXICOLOGY}. ABINGDON: TAYLOR \&{} FRANCIS LTD, 2020, roč.~14, č.~2, s.~214-231. ISSN~1743-5390. Dostupné z: https://dx.doi.org/10.1080/17435390.2019.1685696.

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