KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK a Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS. TOTOWA: HUMANA PRESS INC, 2019, roč. 2035, č. 2019, s. 397-405. ISSN 1064-3745. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-9666-7_25. |
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@article{1643296, author = {Krafčíková, Michaela and HanselandHertsch, R. and Trantírek, Lukáš and Trantírková, Silvie}, article_location = {TOTOWA}, article_number = {2019}, doi = {http://dx.doi.org/10.1007/978-1-4939-9666-7_25}, keywords = {In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex}, language = {eng}, issn = {1064-3745}, journal = {G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS}, title = {In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes}, url = {https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25}, volume = {2035}, year = {2019} }
TY - JOUR ID - 1643296 AU - Krafčíková, Michaela - Hansel-Hertsch, R. - Trantírek, Lukáš - Trantírková, Silvie PY - 2019 TI - In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes JF - G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS VL - 2035 IS - 2019 SP - 397-405 EP - 397-405 PB - HUMANA PRESS INC SN - 10643745 KW - In-cell NMR KW - Xenopus laevis oocyte KW - DNA KW - RNA KW - G-quadruplex UR - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25 L2 - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25 N2 - G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy. ER -
KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK a Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. \textit{G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS}. TOTOWA: HUMANA PRESS INC, 2019, roč.~2035, č.~2019, s.~397-405. ISSN~1064-3745. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-9666-7\_{}25.
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