In Cell NMR Spectroscopy: Investigation of G-Quadruplex
Structures Inside Living Xenopus laevis Oocytes

J 2019

In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes

KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK and Silvie TRANTÍRKOVÁ

Basic information

Original name

In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes

Authors

KRAFČÍKOVÁ, Michaela (703 Slovakia, belonging to the institution), R. HANSEL-HERTSCH, Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution) and Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution)

Edition

G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS, TOTOWA, HUMANA PRESS INC, 2019, 1064-3745

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/19:00108250

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/978-1-4939-9666-7_25

UT WoS

000520558900026

Keywords in English

In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex

Abstract

V originále

G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.

Links

GA17-12075S, research and development project

Name: Polymorfní G-quadruplexy v promotorových oblastech genů

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

653706, interní kód MU

Name: iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research (Acronym: iNEXT)

Investor: European Union, iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research, RI Research Infrastructures (Excellent Science)

Citovat

KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK and Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS. TOTOWA: HUMANA PRESS INC, 2019, vol. 2035, No 2019, p. 397-405. ISSN 1064-3745. Available from: https://dx.doi.org/10.1007/978-1-4939-9666-7_25.

@article{1643296,
   author = {Krafčíková, Michaela and HanselandHertsch, R. and Trantírek, Lukáš and Trantírková, Silvie},
   article_location = {TOTOWA},
   article_number = {2019},
   doi = {http://dx.doi.org/10.1007/978-1-4939-9666-7_25},
   keywords = {In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex},
   language = {eng},
   issn = {1064-3745},
   journal = {G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS},
   title = {In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes},
   url = {https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25},
   volume = {2035},
   year = {2019}
}

TY  - JOUR
ID  - 1643296
AU  - Krafčíková, Michaela - Hansel-Hertsch, R. - Trantírek, Lukáš - Trantírková, Silvie
PY  - 2019
TI  - In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes
JF  - G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS
VL  - 2035
IS  - 2019
SP  - 397-405
EP  - 397-405
PB  - HUMANA PRESS INC
SN  - 10643745
KW  - In-cell NMR
KW  - Xenopus laevis oocyte
KW  - DNA
KW  - RNA
KW  - G-quadruplex
UR  - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25
L2  - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25
N2  - G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.
ER  -

KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK and Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. \textit{G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS}. TOTOWA: HUMANA PRESS INC, 2019, vol.~2035, No~2019, p.~397-405. ISSN~1064-3745. Available from: https://dx.doi.org/10.1007/978-1-4939-9666-7\_{}25.

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