The depletion of p38alpha kinase upregulates NADPH oxidase
2/NOX2/gp91 expression and the production of superoxide in
mouse embryonic stem cells

J 2019

The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK et. al.

Základní údaje

Originální název

The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells

Autoři

BINÓ, Lucia (703 Slovensko, domácí), Iva VESELÁ (203 Česká republika, domácí), Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ (203 Česká republika), Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ (203 Česká republika, domácí), Jan KUČERA (203 Česká republika, domácí), Markéta HANÁČKOVÁ (203 Česká republika, domácí), Lukáš KUBALA (203 Česká republika) a Jiří PACHERNÍK (203 Česká republika, garant, domácí)

Vydání

Archives of Biochemistry and Biophysics, Elsevier Inc. 2019, 0003-9861

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.391

Kód RIV

RIV/00216224:14310/19:00108263

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.abb.2019.06.001

UT WoS

000525438900003

Klíčová slova anglicky

Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2024 08:16, Mgr. Marie Šípková, DiS.

Anotace

V originále

P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.

Návaznosti

GA17-05466S, projekt VaV

Název: Role kanonické signální dráhy Wnt v neurogenezi. (Akronym: WntNeuro)

Investor: Grantová agentura ČR, Role kanonické signální dráhy Wnt v neurogenezi.

MUNI/A/1145/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Akronym: OFIŽ 2018)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA a Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. Archives of Biochemistry and Biophysics. Elsevier Inc., 2019, roč. 671, August 2019, s. 18-26. ISSN 0003-9861. Dostupné z: https://dx.doi.org/10.1016/j.abb.2019.06.001.

@article{1645656,
   author = {Binó, Lucia and Veselá, Iva and Papežíková, Iva and Procházková, Jiřina and Vašíček, Ondřej and Štefková, Kateřina and Kučera, Jan and Hanáčková, Markéta and Kubala, Lukáš and Pacherník, Jiří},
   article_number = {August 2019},
   doi = {http://dx.doi.org/10.1016/j.abb.2019.06.001},
   keywords = {Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase},
   language = {eng},
   issn = {0003-9861},
   journal = {Archives of Biochemistry and Biophysics},
   title = {The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0003986118303813},
   volume = {671},
   year = {2019}
}

TY  - JOUR
ID  - 1645656
AU  - Binó, Lucia - Veselá, Iva - Papežíková, Iva - Procházková, Jiřina - Vašíček, Ondřej - Štefková, Kateřina - Kučera, Jan - Hanáčková, Markéta - Kubala, Lukáš - Pacherník, Jiří
PY  - 2019
TI  - The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells
JF  - Archives of Biochemistry and Biophysics
VL  - 671
IS  - August 2019
SP  - 18-26
EP  - 18-26
PB  - Elsevier Inc.
SN  - 00039861
KW  - Embryonic stem cell
KW  - p38 kinase
KW  - Reactive oxygen species
KW  - NADPH oxidase
UR  - https://www.sciencedirect.com/science/article/pii/S0003986118303813
L2  - https://www.sciencedirect.com/science/article/pii/S0003986118303813
N2  - P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
ER  -

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA a Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. \textit{Archives of Biochemistry and Biophysics}. Elsevier Inc., 2019, roč.~671, August 2019, s.~18-26. ISSN~0003-9861. Dostupné z: https://dx.doi.org/10.1016/j.abb.2019.06.001.

Podrobný výpis o publikaci