Capillary electrophoresis-based immunoassay and aptamer assay:
A review

J 2020

Capillary electrophoresis-based immunoassay and aptamer assay: A review

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ

Základní údaje

Originální název

Capillary electrophoresis-based immunoassay and aptamer assay: A review

Autoři

NEVÍDALOVÁ, Hana (203 Česká republika, domácí), Lenka MICHALCOVÁ (203 Česká republika, domácí) a Zdeněk GLATZ (203 Česká republika, garant, domácí)

Vydání

Electrophoresis, Hoboken, Wiley, 2020, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.535

Kód RIV

RIV/00216224:14310/20:00114103

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/elps.201900426

UT WoS

000511222500001

Klíčová slova anglicky

Application; Aptamer; CE; Immunoassay; Microchip

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 11. 2020 14:56, Mgr. Marie Šípková, DiS.

Anotace

ORIG CZ

V originále

Over the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.

Česky

Článek je zaměřen na bopanalytická stanovení pomocí CE a afinitních značek - protilátek a aptamerů.

Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Citovat

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ. Capillary electrophoresis-based immunoassay and aptamer assay: A review. Electrophoresis. Hoboken: Wiley, 2020, roč. 41, 7-8, s. 414-433. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900426.

@article{1646356,
   author = {Nevídalová, Hana and Michalcová, Lenka and Glatz, Zdeněk},
   article_location = {Hoboken},
   article_number = {7-8},
   doi = {http://dx.doi.org/10.1002/elps.201900426},
   keywords = {Application; Aptamer; CE; Immunoassay; Microchip},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Capillary electrophoresis-based immunoassay and aptamer assay: A review},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.201900426},
   volume = {41},
   year = {2020}
}

TY  - JOUR
ID  - 1646356
AU  - Nevídalová, Hana - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2020
TI  - Capillary electrophoresis-based immunoassay and aptamer assay: A review
JF  - Electrophoresis
VL  - 41
IS  - 7-8
SP  - 414-433
EP  - 414-433
PB  - Wiley
SN  - 01730835
KW  - Application
KW  - Aptamer
KW  - CE
KW  - Immunoassay
KW  - Microchip
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.201900426
L2  - https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.201900426
N2  - Over the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.
ER  -

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ. Capillary electrophoresis-based immunoassay and aptamer assay: A review. \textit{Electrophoresis}. Hoboken: Wiley, 2020, roč.~41, 7-8, s.~414-433. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900426.

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