Application of an electrochemical LAMP-based assay for
screening of HPV16/HPV18 infection in cervical samples

J 2020

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA a Martin BARTOSIK

Základní údaje

Originální název

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Autoři

ANTON, Milan (203 Česká republika, domácí), Ludmila MORÁŇOVÁ (203 Česká republika), Roman HRSTKA (203 Česká republika) a Martin BARTOSIK (203 Česká republika, garant)

Vydání

Analytical Methods, CAMBRIDGE, Royal Society of Chemistry, 2020, 1759-9660

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10401 Organic chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.896

Kód RIV

RIV/00216224:14110/20:00115637

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1039/c9ay02383f

UT WoS

000514662700008

Klíčová slova anglicky

HUMAN-PAPILLOMAVIRUS; HPV TEST; RISK; PLATFORM; ATHENA; ACIDS

Štítky

14110411, GPK, GPKonko, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 7. 2020 09:39, Mgr. Tereza Miškechová

Anotace

V originále

Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value <0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.

Citovat

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA a Martin BARTOSIK. Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples. Analytical Methods. CAMBRIDGE: Royal Society of Chemistry, 2020, roč. 12, č. 6, s. 822-829. ISSN 1759-9660. Dostupné z: https://dx.doi.org/10.1039/c9ay02383f.

@article{1655397,
   author = {Anton, Milan and Moráňová, Ludmila and Hrstka, Roman and Bartosik, Martin},
   article_location = {CAMBRIDGE},
   article_number = {6},
   doi = {http://dx.doi.org/10.1039/c9ay02383f},
   keywords = {HUMAN-PAPILLOMAVIRUS; HPV TEST; RISK; PLATFORM; ATHENA; ACIDS},
   language = {eng},
   issn = {1759-9660},
   journal = {Analytical Methods},
   title = {Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples},
   url = {https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1655397
AU  - Anton, Milan - Moráňová, Ludmila - Hrstka, Roman - Bartosik, Martin
PY  - 2020
TI  - Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples
JF  - Analytical Methods
VL  - 12
IS  - 6
SP  - 822-829
EP  - 822-829
PB  - Royal Society of Chemistry
SN  - 17599660
KW  - HUMAN-PAPILLOMAVIRUS
KW  - HPV TEST
KW  - RISK
KW  - PLATFORM
KW  - ATHENA
KW  - ACIDS
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract
L2  - https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract
N2  - Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value <0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.
ER  -

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA a Martin BARTOSIK. Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples. \textit{Analytical Methods}. CAMBRIDGE: Royal Society of Chemistry, 2020, roč.~12, č.~6, s.~822-829. ISSN~1759-9660. Dostupné z: https://dx.doi.org/10.1039/c9ay02383f.

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